partsregistry.org - Recent changes [en]

Help:Contents

2012-05-16T18:25:06Z

←Older revision		Revision as of 18:25, 16 May 2012
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	<tr><td colspan="3">		<tr><td colspan="3">
	<div id="splash-desc">		<div id="splash-desc">
-	New to iGEM and the Registry? You'll want to head over to the <a href="http://partsregistry.org/Help:Learn">Learn</a> section first to get an introduction on synthetic biology based on standardized parts and the philosophy of iGEM and the Registry. We welcome suggestions via email (<strong>hq (at) igem . org</strong>) or on <a href="http://partsregistry.org/forum/Forum_List?n=Registry">the forum</a>. You can also look through the <a href="http://partsregistry.org/Help:Archive">old help pages</a> if you couldn't find what you're looking for.	+	New to iGEM and the Registry? You'll want to head over to the <a href="http://partsregistry.org/Help:Learn">Learn</a> section first to get an introduction on synthetic biology based on standardized parts and the philosophy of iGEM and the Registry. We welcome suggestions via email (<strong>hq (at) igem . org</strong>) or on <a href="http://partsregistry.org/forum/Forum_List?n=Registry">the forum</a>. You can also look through the <a href="http://partsregistry.org/Help/Archive">old help pages</a> if you couldn't find what you're looking for.
	</div>		</div>
	</td></tr>		</td></tr>

Talk:Registry API

2012-05-16T13:04:15Z

New page: Part BBa_E71204 does not contain a correct XML format. http://partsregistry.org/cgi/xml/part.cgi?part=BBa_E71204 --~~~~

New page

Part BBa_E71204 does not contain a correct XML format.
http://partsregistry.org/cgi/xml/part.cgi?part=BBa_E71204
--[[User:Jjkoehorst|Jjkoehorst]] 13:04, 16 May 2012 (UTC)

Part:BBa M39201:Design

2012-05-16T01:27:35Z

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	__NOTOC__		__NOTOC__
	<partinfo>BBa_M39201 short</partinfo>		<partinfo>BBa_M39201 short</partinfo>
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	===Design Notes===		===Design Notes===
-	The protein had to still be long enough to retain function.	+	The protein had to still be long enough to retain function. The sequence was used from US Patent 7892824.

Part:BBa M39413

2012-05-15T22:26:23Z

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-
	__NOTOC__		__NOTOC__
	<partinfo>BBa_M39413 short</partinfo>		<partinfo>BBa_M39413 short</partinfo>

-	pCat promoter ([[BBa_I14033]]) flanked by regions ([[BBa_K137010]], [[BBa_K137008]]) recognized by fimE recombinase ([[BBa_K137007]]).	+	pCat promoter ([[Part:BBa_I14033\|BBa_I14033]]) flanked by regions ([[Part:BBa_K137010\|BBa_K137010]], [[Part:BBa_K137008\|BBa_K137008]]) recognized by fimE recombinase ([[Part:BBa_K137007\|BBa_K137007]]).

-	pCat is a constitutive promoter, which enables this device to be used as a switch to constitutively express two different regions. By default, the ~~upstream~~ sense strand is promoted. When flipped, the ~~downstream~~ antisense strand is promoted.	+	pCat is a constitutive promoter, which enables this device to be used as a switch to constitutively express two different regions. By default, the downstream sense strand is promoted as usual. When flipped, the upstream antisense strand is promoted.

-	Additionally, ~~downstream~~ of the promoter is a terminating sequence [[BBa_B0015]]. This sequence is to prevent "backwards" transcription before the promoter is flipped. It is between the recombinant regions, causing it to flip with the promoter.	+	Additionally, upstream of the promoter is a terminating sequence [[Part:BBa_B0015\|BBa_B0015]]. This sequence is to prevent "backwards" transcription before the promoter is flipped. It is between the recombinant regions, causing it to flip with the promoter.

	pCat can be replaced with another promoter for additional regulation if needed.		pCat can be replaced with another promoter for additional regulation if needed.

Part:BBa K611066

2012-05-15T19:23:15Z

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-
	__NOTOC__		__NOTOC__
	<partinfo>BBa_K611066 short</partinfo>		<partinfo>BBa_K611066 short</partinfo>

-	This part is designed for testing the promoter region in the barcode region of cI Lambda (BBa_C0051). The barcode has been removed, but the rest of the BBa_C0051 biobrick has been unchanged.	+	This part is designed for testing the promoter region in the barcode region of cI Lambda (BBa_C0051). The barcode has been removed, but the rest of the BBa_C0051 biobrick has been unchanged. It has been placed upstream of an RBS (BBa_B0034) and RFP (BBa_E1010) in the BBa_J61002 plasmid.

	<!-- Add more about the biology of this part here		<!-- Add more about the biology of this part here

Part:BBa K611061

2012-05-15T17:49:54Z

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	__NOTOC__		__NOTOC__
	<partinfo>BBa_K611061 short</partinfo>		<partinfo>BBa_K611061 short</partinfo>

-	test ~~con J61002~~	+	This part is a test construct for the characterization of promoter activity in the barcode region of BBa_C0051.

	<!-- Add more about the biology of this part here		<!-- Add more about the biology of this part here

Part:BBa M39023:Design

2012-05-15T15:48:39Z

References:

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	__NOTOC__		__NOTOC__
	<partinfo>BBa_M39023 short</partinfo>		<partinfo>BBa_M39023 short</partinfo>
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	===Design Notes===		===Design Notes===
-	n/a	+	We needed a way to make sure that E.coli placed in the lungs of CF patients will die upon leaving that environment. To this end, we decided on using an AND gate structure to make sure we have strict regulation needing two signals. One signal is the Sialic acid. This is found in mucosal areas which include the lungs. To narrow down the areas which we want, we added the necessity for another signal, LasL. This is a quorum sensing molecule used by the bacteria causing the infection. With the necessity for these two signals, we greatly narrow the conditions of where this E.coli can survive, leaving only really the lung environment for CF patients.
-		+

		+	The mechanism of death is in the absence of any of the two signals, Granulysin and DNase1 will be produce which will kill the cell.

	===Source===		===Source===

-	E.coli	+	E.coli and other organisms

	===References===		===References===
		+	AND gate documentation:
		+	http://www.nature.com/ncomms/journal/v2/n10/full/ncomms1516.html

Help:Distribution Kits

2012-05-15T15:22:27Z

←Older revision		Revision as of 15:22, 15 May 2012
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	<tr>		<tr>
	<td colspan="2" style="vertical-align:text-top;">		<td colspan="2" style="vertical-align:text-top;">
-	Before you start using ~~the~~ distribution kit, make sure to read the instructions!	+	Before you start using your distribution kit, make sure to read through the instructions!
	</td>		</td>
	</tr>		</tr>
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	</html>		</html>
	{{TOCright}}		{{TOCright}}
-	The 2012 Distribution contains over 1000 part samples as dried (miniprepped) DNA, with each sample [[Help:Quality_Control\|QC tested]] through [[Help:Quality_Control#Sequencing_Results\|sequencing]], [[Help:Quality_Control#Antibiotic_Test_Plates\|AB test plates]], and [[Help:Quality_Control#Gel_Results\|restriction digests]]. While there is not enough DNA for assembly, you will be able to [[Help:Distribution_Kits#DNA Kit Plate Instruction\|transform the DNA]] into cells and then make your own glycerol stocks of any part ~~that~~ you wish. This year's distribution does not contain all of the part samples available in the [[Help:Repository\|Registry's Repository]], but it does include both high-quality part samples from the past as well as some of the 2012 team favorites and highly requested parts during the 2011 season.	+	The 2012 Distribution contains over 1000 part samples as dried (miniprepped) DNA, with each sample [[Help:Quality_Control\|QC tested]] through [[Help:Quality_Control#Sequencing_Results\|sequencing]], [[Help:Quality_Control#Antibiotic_Test_Plates\|AB test plates]], and [[Help:Quality_Control#Gel_Results\|restriction digests]]. While there is not enough DNA for assembly, you will be able to [[Help:Distribution_Kits#DNA Kit Plate Instruction\|transform the DNA]] into cells and then make your own glycerol stocks of any part you wish. This year's distribution does not contain all of the part samples available in the [[Help:Repository\|Registry's Repository]], but it does include both high-quality part samples from the past as well as some of the 2012 team favorites and highly requested parts during the 2011 season.

	==What's included in the 2012 Distribution==		==What's included in the 2012 Distribution==
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	==Getting Started==		==Getting Started==
-	If you're new to the Registry, iGEM, or synthetic biology, you'll want to read through our Help ~~page~~ before you get started. The [[Help:Learn\|Learn section]] is the best place to start, and will take you through the basics.	+	If you're new to the Registry, iGEM, or synthetic biology, you'll want to read through our Help pages before you get started. The [[Help:Learn\|Learn section]] is the best place to start, and will take you through the basics.

	Otherwise, <b>read on!</b>		Otherwise, <b>read on!</b>

Help:Protocols/Linearized Plasmid Backbones

2012-05-15T14:58:39Z

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-	{{~~TOCright~~}}	+	__NOTOC__
-	~~''This protocol was developed by Tom Knight, samples of standard Registry plasmid backbones prepared using this method were sent out in the [[Help~~:~~Spring 2011 DNA distribution \| Spring 2011 DNA Distribution kits]].''~~	+	{{HelpPage/MainCSS}}
		+	<html>
		+	<style type="text/css">
		+	#mm-help img {opacity: 1}

-	Short single stranded DNA fragments will not ligate to 4 bp overhangs. By creating a very short overhang on a PCR of a plasmid backbone, the remnant, when cut with EcoRI and PstI is sufficiently short that it will not anneal at ligation temperature, and will therefore not ligate. This allows us to build high quality construction plasmid backbone without purifying away the cut fragments remaining after PCR.	+	</style>

-	~~We are distributing the prepared construction~~ plasmid ~~as purified PCR products, diluted to standard concentration, but prior to cutting with EcoRI~~ and ~~PstI~~. ~~Standard assembly will cut this plasmid backbone with EcoRI and PstI at~~ the ~~same time that~~ the ~~two assembled fragments are cut with EcoRI and SpeI and with XbaI~~ and ~~PstI, respectively~~.	+	<table id="nav-table">
		+	<tr><td colspan="3"></html>{{HelpPage/MiniMap}}<html></td></tr>
		+	<tr>
		+	<td style="width: 120px; height: 100px;" rowspan="2"></td>
		+	<td colspan="2"><div id="splash-title">Linearized Plasmid Backbones</div></td>
		+	</tr>
		+	<tr>
		+	<td colspan="2" style="vertical-align:text-top;">
		+	iGEM HQ provides linearized plasmid backbones for use during 3A Assembly and for shipping parts (pSB1C3). The following are the recommended protocols for using the linearized backbones and making your own.
		+	</td>
		+	</tr>
		+	</table>

-	The preparation of this PCR fragment is done with primers having short overhangs past the EcoRI and PstI sites, followed by PCR cleanup, dilution to standard concentration, and quality control testing.	+	</html>

-	~~<span style=~~'~~color:red; font-weight:bold;~~'~~>Note:</span> The~~ Registry [[DNA ~~Submission Instructions~~ \| ~~shipping plasmid backbone~~]] is ''~~'pSB1C3'''. If you are making linearized plasmid backbone in order to send parts to the Registry, you must use pBS1C3.~~	+	{{TOCright}}
		+	''This protocol was developed by Tom Knight. Samples of standard Registry plasmid backbones prepared using this method were sent out in the [[Help:Spring 2011 DNA distribution \| Spring 2011 DNA Distribution kits]].''

-		+	==Why Linearized Plasmid Backbones?==
-	~~'''Primers:'''~~	+	Short single stranded DNA fragments will not ligate to 4 bp overhangs. By creating a very short overhang on a PCR of a plasmid backbone, the remnant, when cut with EcoRI and PstI is sufficiently short that it will not anneal at ligation temperature, and will therefore not ligate. This allows us to build high quality construction plasmid backbone without purifying away the cut fragments remaining after PCR.
-	~~gccgctgcagtccggcaaaaaa~~,~~SB-prep-3P-1~~	+
-	~~atgaattccagaaatcatccttagcg~~,~~SB-prep-2Ea~~	+
-		+
-	~~Diluted to 30 pmol/ul~~	+
-		+
-	~~These primers have been tested~~ with ~~pSB1C3, pSB1A3, pSB1K3~~, and ~~pSB1T3~~.	+


	=Using the Linearized Plasmid Backbones=		=Using the Linearized Plasmid Backbones=
-	The Spring ~~2011~~ DNA Distribution should come with a set of linearized plasmid backbones: pSB1A3, pSB1C3, pSB1K3, and pSB1T3. The linearized plasmid backbones (25ng/ul at 50ul) should be stored at 4C or lower. Prior to ligation the plasmid backbones need to be cut with EcoRI and PstI.	+	The Spring 2012 DNA Distribution should come with a set of linearized plasmid backbones: pSB1A3, pSB1C3, pSB1K3.m1, and pSB1T3. The linearized plasmid backbones (25ng/ul at 50ul) should be stored at 4C or lower. Prior to ligation the plasmid backbones need to be cut with EcoRI and PstI.

	==Digest==		==Digest==
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	[[Help:Production/2012 Plasmid Backbones\| 2012 Plasmid Backbone Production]]		[[Help:Production/2012 Plasmid Backbones\| 2012 Plasmid Backbone Production]]
		+
	[[2011 Plasmid Backbone Production]]		[[2011 Plasmid Backbone Production]]

	===PCR mix===		===PCR mix===
		+	====Primers====
		+	gccgctgcagtccggcaaaaaa,SB-prep-3P-1
		+	atgaattccagaaatcatccttagcg,SB-prep-2Ea
		+
		+	Diluted to 30 pmol/ul
		+
		+	These primers have been tested with pSB1C3, pSB1A3, pSB1K3, and pSB1T3.
		+
		+	====Bulk Reaction====
	* 9.6ml of [http://products.invitrogen.com/ivgn/product/10790020?ICID=search-10790020 PCR Supermix High Fidelity]		* 9.6ml of [http://products.invitrogen.com/ivgn/product/10790020?ICID=search-10790020 PCR Supermix High Fidelity]
	* 67 ul of primer SB-prep-2Eb		* 67 ul of primer SB-prep-2Eb
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	[[Linearized Plasmid Backbones Original Protocol]]		[[Linearized Plasmid Backbones Original Protocol]]
		+
		+
		+	{{HelpPage/Footer}}

Registry:Bug reports

2012-05-15T14:54:11Z

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		+	===XML Data incorrect===
		+	The XML data that can be retrieved from the front page does not show consistency. For example the sub parts, 940992 with part_id 4901 and subpart 2006301 with part_id 14010 do not have a parent in the parts table. When working with foreign keys ±6000 entry's cannot be inserted as the parent part_id is not present. Are there other sources available for consistent retrieval?
		+	[[User:JJkoehorst\|JJkoehorst]] 15:32, 5 May 2012
		+
		+	----
	===BARCODES NOT BEING REPRESENTED!!===		===BARCODES NOT BEING REPRESENTED!!===

Talk:Main Page

2012-05-15T13:58:04Z

←Older revision		Revision as of 13:58, 15 May 2012
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	How does one properly cite the Registry? It would be nice to see this info posted somewhere.		How does one properly cite the Registry? It would be nice to see this info posted somewhere.
	--[[User:Kahaynes\|Kahaynes]] 15:30, 13 January 2007 (EST)		--[[User:Kahaynes\|Kahaynes]] 15:30, 13 January 2007 (EST)
		+
		+	Is it possible to have an update dump file in an SQL format? --[[User:jjkoehorst\|jjkoehorst]] 14:57, 15 May 2012

Help:Distribution Kits

2012-05-14T22:05:34Z

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	==What's included in the 2012 Distribution==		==What's included in the 2012 Distribution==
-	[[Image:~~2011-Distribution-Contents~~.jpg‎\|500px\|center]]	+	[[Image:2012_Distribution.jpg‎\|500px\|center]]

	Your Spring 2012 Distribution contains the following:		Your Spring 2012 Distribution contains the following:

Special:Log/upload

2012-05-14T22:04:31Z

uploaded "Image:2012 Distribution.jpg"

Help:Production/2012 Plasmid Backbones

2012-05-14T21:49:35Z

←Older revision		Revision as of 21:49, 14 May 2012
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	Sequence: Confirmed		Sequence: Confirmed

		+	[[Image:1C3-B003-1-2.jpg]]
		+	[[Image:1C3-B003-2-2.jpg]]

	===pSB1A3===		===pSB1A3===
	Sequence: Confirmed		Sequence: Confirmed

		+	[[Image:1A3-B002-1-2.jpg]]
		+	[[Image:1A3-B002-2-2.jpg]]

	===pSB1T3===		===pSB1T3===
	Sequence: Confirmed		Sequence: Confirmed

		+	[[Image:1T3-B002-1-2.jpg]]
		+	[[Image:1T3-B002-2-2.jpg]]

	===pSB1K3.m1===		===pSB1K3.m1===
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	The 2012 Distribution Kit also includes a mutant version of pSB1K3. This mutant version has a sbp mutation on its VR site. While the mutation will not affect the function of the plasmid, it may affect sequencing using the VR primer. '''Note:''' This version of pSB1K3 was sequenced using the correct VR primer three times. All three times, the primer annealed to the correct reading and gave an accurate sequencing read.		The 2012 Distribution Kit also includes a mutant version of pSB1K3. This mutant version has a sbp mutation on its VR site. While the mutation will not affect the function of the plasmid, it may affect sequencing using the VR primer. '''Note:''' This version of pSB1K3 was sequenced using the correct VR primer three times. All three times, the primer annealed to the correct reading and gave an accurate sequencing read.
		+
		+	[[Image:1K3m1-B001-1-2.jpg]]
		+	[[Image:1K3m1-B001-2-2.jpg]]

Special:Log/upload

2012-05-14T21:43:36Z

uploaded "Image:1T3-B002-2-2.jpg"

Special:Log/upload

2012-05-14T21:43:27Z

uploaded "Image:1T3-B002-1-2.jpg"

Special:Log/upload

2012-05-14T21:43:16Z

uploaded "Image:1K3m1-B001-2-2.jpg"

Special:Log/upload

2012-05-14T21:43:07Z

uploaded "Image:1K3m1-B001-1-2.jpg"

Special:Log/upload

2012-05-14T21:42:56Z

uploaded "Image:1C3-B003-2-2.jpg"

Special:Log/upload

2012-05-14T21:42:44Z

uploaded "Image:1C3-B003-1-2.jpg"

Special:Log/upload

2012-05-14T21:40:50Z

uploaded "Image:1A3-B002-2-2.jpg"

Special:Log/upload

2012-05-14T21:40:41Z

uploaded "Image:1A3-B002-1-2.jpg"

Help:Production/2012 Plasmid Backbones

2012-05-14T21:18:02Z

←Older revision		Revision as of 21:18, 14 May 2012
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	===pSB1C3===		===pSB1C3===
		+	Sequence: Confirmed
		+

	===pSB1A3===		===pSB1A3===
		+	Sequence: Confirmed
		+

	===pSB1T3===		===pSB1T3===
		+	Sequence: Confirmed
		+

	===pSB1K3.m1===		===pSB1K3.m1===
		+	Sequence: Inconsistent
		+
	The 2012 Distribution Kit also includes a mutant version of pSB1K3. This mutant version has a sbp mutation on its VR site. While the mutation will not affect the function of the plasmid, it may affect sequencing using the VR primer. '''Note:''' This version of pSB1K3 was sequenced using the correct VR primer three times. All three times, the primer annealed to the correct reading and gave an accurate sequencing read.		The 2012 Distribution Kit also includes a mutant version of pSB1K3. This mutant version has a sbp mutation on its VR site. While the mutation will not affect the function of the plasmid, it may affect sequencing using the VR primer. '''Note:''' This version of pSB1K3 was sequenced using the correct VR primer three times. All three times, the primer annealed to the correct reading and gave an accurate sequencing read.

Help:Resources

2012-05-14T21:14:23Z

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		+	{{HelpPage/MainCSS}}
	<html>		<html>
	<style type="text/css">		<style type="text/css">
-	~~.firstHeading~~ {~~display~~: none;}	+	ul#splash-list, #splash-list li {font-size:11pt; list-style: none; margin: 0px 40px 25px 40px;}
-	.splash .~~title1~~ {~~font~~-~~family~~: ~~"Helvetica", Helvetica, sans~~-~~serif~~; font-~~weight~~: ~~bold~~; font-~~size~~:~~20pt~~; color: #006633; ~~display~~: ~~inline~~;}	+	#splash-list li {display: block; position: relative; padding:5px; min-height: 101px;}
-	.~~splash li~~ {font-size:~~11pt~~; ~~list~~-~~style~~: ~~none~~; ~~width~~: ~~700px~~; margin: ~~30px~~ 0px ~~0px 40px~~;}	+	.list-table {background: none;}
-	.~~splash li~~ img {~~display~~: ~~inline; width: 25px~~; ~~margin~~-~~top~~: ~~-10px~~; ~~margin~~-~~right~~: ~~5px~~;}	+	.list-icon {vertical-align: top; padding-right: 5px;}
		+	.list-title a {font-size:15pt; font-weight: bold; color: #006633;}
		+	.list-title a:hover {color: #339933;}
		+	.list-desc {width: 100%; padding: 5px;}
		+	.quicklinks {vertical-align: bottom; font-size: 10pt; font-weight: bold; color: #3366FF; margin-bottom: 0px;}
		+	.list-icon img {opacity: 1;}
		+	.list-icon img:hover {opacity: .45;}
		+
		+
		+	#mm-help img {opacity: 1;}
	</style>		</style>
	<body>		<body>
-	<div ~~class~~="splash">	+	<table id="splash-body">
-	<p><div ~~class~~="~~title1~~">~~RESOURCES~~</div>	+	<tr><td>
-	<br>	+	</html>
-	Lorem ipsum dolor sit amet, usu an justo integre fierent, duo tota nobis cu. Viris patrioque an est, utinam maiorum commune in eos, eu elitr interpretaris nec. Ut mei suas antiopam, modus suavitate ex mei, usu et sale prima graecis. Mea ad deserunt comprehensam, ullum errem appetere at eam, ius zril complectitur ei. Ea noluisse delectus euripidis vel.</p>	+	{{HelpPage/MiniMap}}
-	<br>	+	<html>
		+	</td></tr>
		+
		+	<tr><td><div id="splash-title">Resources</div></td></tr>
		+
		+	<tr><td>
		+	<div id="splash-desc">
		+
		+	</div>
		+	</td></tr>
		+
		+	<tr><td>
		+
		+	</td></tr>
		+
		+	<tr><td>

		+	<ul id="splash-list">
	<li>		<li>
-	<~~div~~ class="~~title1~~"><~~a style~~="~~color:#006633~~" href="http://partsregistry.org/Registry_FAQ">frequently asked questions</a></~~div~~>	+	<table class="list-table">
-	<br>	+	<tr>
-	Have questions about the Registry and how to use it? We've got answers.	+	<td rowspan="3" class="list-icon"></td>
		+	<td class="list-title"><a href="http://partsregistry.org/Registry_FAQ">frequently asked questions</a></td>
		+	</tr>
		+	<tr><td class="list-desc">Have questions about the Registry and how to use it? We've got answers. </td></tr>
		+	<tr><td class="quicklinks"></td></tr>
		+	</table>
	</li>		</li>

	<li>		<li>
-	<~~div~~ class="~~title1~~"> <~~a style~~="~~color:#006633~~" href="http://partsregistry.org/Help:Glossary">glossary</a></~~div~~>	+	<table class="list-table">
-	<br>	+	<tr>
-	Some common terms you'll come across in the Registry, indexed alphabetically.	+	<td rowspan="3" class="list-icon"></td>
		+	<td class="list-title"><a href="http://partsregistry.org/Help:Glossary">glossary</a></td>
		+	</tr>
		+	<tr><td class="list-desc">Some common terms you'll come across in the Registry, indexed alphabetically. </td></tr>
		+	<tr><td class="quicklinks"></td></tr>
		+	</table>
	</li>		</li>

	<li>		<li>
-	<~~div~~ class="~~title1~~"><~~a style~~="~~color:#006633~~" href="http://partsregistry.org/Help:site_map">site map</a></~~div~~>	+	<table class="list-table">
-	<br>	+	<tr>
-	A guide showing all the pages in the Help section as they are linked to each other.	+	<td rowspan="3" class="list-icon"></td>
		+	<td class="list-title"><a href="http://partsregistry.org/Help:site_map">site map</a></td>
		+	</tr>
		+	<tr><td class="list-desc">A guide showing all the pages in the Help section as they are linked to each other. </td></tr>
		+	<tr><td class="quicklinks"></td></tr>
		+	</table>
	</li>		</li>

	<li>		<li>
-	<~~div~~ class="~~title1~~"> <~~a style~~="~~color:#006633~~" href="http://igem.org/Contact">contact</a></~~div~~>	+	<table class="list-table">
-	<br>	+	<tr>
-	Still have questions? You can always reach iGEM Headquarters via email.	+	<td rowspan="3" class="list-icon"></td>
		+	<td class="list-title"><a href="http://igem.org/Contact">contact</a></td>
		+	</tr>
		+	<tr><td class="list-desc">Still have questions? You can always reach iGEM Headquarters via email. </td></tr>
		+	<tr><td class="quicklinks"></td></tr>
		+	</table>
	</li>		</li>

-	~~<br><br>~~	+
	</ul>		</ul>
-	</~~splash~~>	+
		+	</td></tr>
		+
		+	</table>
	</body>		</body>
	</html>		</html>

Help:Production/2012 Plasmid Backbones

2012-05-14T19:07:19Z

New page: ===pSB1C3=== ===pSB1A3=== ===pSB1T3=== ===pSB1K3.m1=== The 2012 Distribution Kit also includes a mutant version of pSB1K3. This mutant version has a sbp mutation on its VR site. While t...

New page

===pSB1C3===

===pSB1A3===

===pSB1T3===

===pSB1K3.m1===
The 2012 Distribution Kit also includes a mutant version of pSB1K3. This mutant version has a sbp mutation on its VR site. While the mutation will not affect the function of the plasmid, it may affect sequencing using the VR primer. '''Note:''' This version of pSB1K3 was sequenced using the correct VR primer three times. All three times, the primer annealed to the correct reading and gave an accurate sequencing read.

Help:Redesign

2012-05-14T18:58:09Z

←Older revision		Revision as of 18:58, 14 May 2012
Line 22:		Line 22:
	}		}

-	#tier1 {height: ~~200px~~;}	+	#tier1 {height: 180px;}
-	#tier2 {height: ~~200px~~;}	+	#tier2 {height: 100px;}
		+	#tier3 {height: 140px;}

	.catblock .title1 a {font-size:20pt; font-weight: bold; color: #006633;}		.catblock .title1 a {font-size:20pt; font-weight: bold; color: #006633;}
Line 107:		Line 108:
	<tr>		<tr>
	<td>		<td>
-	<div class="catblock" id="~~tier1~~">	+	<div class="catblock" id="tier2">
	<div class="title1"><a href="http://partsregistry.org/Help:Use">use!</a></div>		<div class="title1"><a href="http://partsregistry.org/Help:Use">use!</a></div>
	<ul>		<ul>
Line 116:		Line 117:

	<td>		<td>
-	<div class="catblock" id="~~tier1~~">	+	<div class="catblock" id="tier2">
	<div class="title1"><a href="http://partsregistry.org/Help:Give">give!</a></div>		<div class="title1"><a href="http://partsregistry.org/Help:Give">give!</a></div>
	<ul>		<ul>
Line 125:		Line 126:

	<td>		<td>
-	<div class="catblock" id="~~tier1~~">	+	<div class="catblock" id="tier2">
	<div class="title1"><a href="http://partsregistry.org/Help:Share">share!</a></div>		<div class="title1"><a href="http://partsregistry.org/Help:Share">share!</a></div>
	<ul>		<ul>
Line 136:		Line 137:
	<tr>		<tr>
	<td>		<td>
-	<div class="catblock" id="~~tier2~~">	+	<div class="catblock" id="tier3">
	<div class="title1"><a href="http://partsregistry.org/Help:Nav&Tools">navigation & tools</div>		<div class="title1"><a href="http://partsregistry.org/Help:Nav&Tools">navigation & tools</div>
	<ul>		<ul>
Line 143:		Line 144:
	<li><a href="http://partsregistry.org/Help:Nav/Get_This_Part">get this part page</a></li>		<li><a href="http://partsregistry.org/Help:Nav/Get_This_Part">get this part page</a></li>
	<li><a href="http://partsregistry.org/Help:Nav/Part_Tools">part tools</a></li>		<li><a href="http://partsregistry.org/Help:Nav/Part_Tools">part tools</a></li>
-	<li>more...</li>	+	<li><a href="http://partsregistry.org/Help:Nav&Tools">more...</a></li>
	</ul>		</ul>
	</div>		</div>
Line 149:		Line 150:

	<td>		<td>
-	<div class="catblock" id="~~tier2~~">	+	<div class="catblock" id="tier3">
	<div class="title1"><a href="http://partsregistry.org/Help:Protocol">protocols</a></div>		<div class="title1"><a href="http://partsregistry.org/Help:Protocol">protocols</a></div>
	<ul>		<ul>
-	<li>~~how to transform parts~~</li>	+	<li><a href="http://partsregistry.org/Help:3A_Assembly_Kit">3A Assembly Kit</a></li>
-	<li>~~how to~~ "~~biobrick~~" a ~~part~~</li>	+	<li><a href="http://partsregistry.org/Help:Protocols/Transformation">transformation</a></li>
-	<li>~~how to assemble parts~~</li>	+	<li><a href="http://partsregistry.org/Help:Protocols/Competent_Cells">competent cells</a></li>
-	<li>more...</li>	+	<li><a href="http://partsregistry.org/Help:Protocols/Linearized_Plasmid_Backbones">linearized plasmid backbones</a></li>
		+	<li><a href="http://partsregistry.org/Help:Protocol">more...</a></li>
	</ul>		</ul>
	</div>		</div>
Line 161:		Line 163:

	<td>		<td>
-	<div class="catblock" id="~~tier2~~">	+	<div class="catblock" id="tier3">
	<div class="title1"><a href="http://partsregistry.org/Help:Resources">resources</a></div>		<div class="title1"><a href="http://partsregistry.org/Help:Resources">resources</a></div>
	<ul>		<ul>

Help:Distribution Kits

2012-05-14T18:38:45Z

←Older revision		Revision as of 18:38, 14 May 2012
Line 95:		Line 95:

	You can find more in depth instructions on how to use and make your own linearized plasmid backbones on the [[Help:Protocols/Linearized Plasmid Backbones \| protocol page]]. ''Note: The linearized plasmid backbones need to be cut by EcoRI and PstI restriction enzymes prior to use.''		You can find more in depth instructions on how to use and make your own linearized plasmid backbones on the [[Help:Protocols/Linearized Plasmid Backbones \| protocol page]]. ''Note: The linearized plasmid backbones need to be cut by EcoRI and PstI restriction enzymes prior to use.''
		+
		+
		+	[[Help:Production/2012 Plasmid Backbones\| 2012 Plasmid Backbone Production]]

Help:Protocols/Linearized Plasmid Backbones

2012-05-14T18:37:32Z

←Older revision		Revision as of 18:37, 14 May 2012
Line 53:		Line 53:
	The following is the protocol that we used to create the linearized plasmid backbones shipped with the Spring 2011 DNA Distribution. The protocol is in 96 well format, but may be scaled down to suit smaller batches.		The following is the protocol that we used to create the linearized plasmid backbones shipped with the Spring 2011 DNA Distribution. The protocol is in 96 well format, but may be scaled down to suit smaller batches.

		+	[[Help:Production/2012 Plasmid Backbones\| 2012 Plasmid Backbone Production]]
	[[2011 Plasmid Backbone Production]]		[[2011 Plasmid Backbone Production]]

Help:Protocol

2012-05-14T18:34:23Z

(Difference between revisions)

Help:Distribution Kits

2012-05-14T16:00:22Z

←Older revision		Revision as of 16:00, 14 May 2012
(One intermediate revision not shown.)
Line 28:		Line 28:
	Your Spring 2012 Distribution contains the following:		Your Spring 2012 Distribution contains the following:
	* (5) 384w DNA Distribution Kit Plates		* (5) 384w DNA Distribution Kit Plates
-	* Linearized Plasmid Backbones: pSB1A3, pSB1C3, pSB1K3.m1, pSB1T3	+	* [[Help:Distribution_Kits#Linearized Plasmid Backbones\|Linearized Plasmid Backbones]]: pSB1A3, pSB1C3, pSB1K3.m1, pSB1T3
	* iGEM Stickers and Pins!		* iGEM Stickers and Pins!

Line 34:		Line 34:

	==Getting Started==		==Getting Started==
-	If you're new to the Registry, iGEM, or synthetic biology, you'll want to read through our Help page before you get started. The [[Help:Learn\|Learn section]] is the ~~proper~~ place to start, and will take you through the basics.	+	If you're new to the Registry, iGEM, or synthetic biology, you'll want to read through our Help page before you get started. The [[Help:Learn\|Learn section]] is the best place to start, and will take you through the basics.

-	Otherwise, read on!	+	Otherwise, <b>read on!</b>

	===Locating a Part in the Distribution===		===Locating a Part in the Distribution===
Line 45:		Line 45:
	*The '''[[Help:Locating_Parts\|Search Tools]]''' will let you search by text or part name <more>.		*The '''[[Help:Locating_Parts\|Search Tools]]''' will let you search by text or part name <more>.
	*It is also possible to see the contents of the Kit Plates in their entirety. You can look at the [http://partsregistry.org/cgi/assembly/libraries.cgi DNA Repositories] section on the main page.		*It is also possible to see the contents of the Kit Plates in their entirety. You can look at the [http://partsregistry.org/cgi/assembly/libraries.cgi DNA Repositories] section on the main page.
-	**Click on Spring 2012 Distribution to see the contents of all of your kit plates.	+	**Click on [http://partsregistry.org/assembly/libraries.cgi?id=42 Spring 2012 Distribution] to see the contents of all of your kit plates.
	**Click on the 2012 Kit Plate of your choice, which will list all parts by their part name (BBa_..) in a plate along with their quality control information. Or you can click on the small part diagram below each Kit Plate link: "See a summary of the parts in this plate."		**Click on the 2012 Kit Plate of your choice, which will list all parts by their part name (BBa_..) in a plate along with their quality control information. Or you can click on the small part diagram below each Kit Plate link: "See a summary of the parts in this plate."
	**These options will show you what is in each well of your plate, however they are not the best way to find specific parts you would like to use.		**These options will show you what is in each well of your plate, however they are not the best way to find specific parts you would like to use.
Line 55:		Line 55:
	If you find a part that you would like to use, you need to make sure that a sample of the part is actually available. There are many parts in the Registry that people are still working on, or decided not to continue working on anymore, therefore we never received or do not yet have the physical DNA for them. This of course means that the DNA is not available in the Spring 2012 DNA Distribution. The simplest way to tell whether the part has an available sample is to look at the top right of the part's Main Page. If the part has an available sample the top part of the box will be green and say "DNA available."		If you find a part that you would like to use, you need to make sure that a sample of the part is actually available. There are many parts in the Registry that people are still working on, or decided not to continue working on anymore, therefore we never received or do not yet have the physical DNA for them. This of course means that the DNA is not available in the Spring 2012 DNA Distribution. The simplest way to tell whether the part has an available sample is to look at the top right of the part's Main Page. If the part has an available sample the top part of the box will be green and say "DNA available."

-	===~~Ordering~~ a part===	+	===Requesting a part===
-	We've taken care to create a very well-rounded DNA Distribution this spring, however, should you find the part, or parts, that you require are not available in the distribution but available in the Registry, ~~feel free to~~ send us an email (hq [AT] igem [DOT] org) in order to request a sample. Just include the part name, the plasmid it's located in, and the source, and we'll send it out to you. For more information please see the [[Help:Requesting_Parts\|Requesting Parts page]]	+	We've taken care to create a very well-rounded DNA Distribution this spring, however, should you find the part, or parts, that you require are not available in the distribution but available in the Registry's Repository, send us an email (hq [AT] igem [DOT] org) in order to request a sample. Just include the part name, the plasmid it's located in, and the source, and we'll send it out to you. For more information please see the [[Help:Requesting_Parts\|Requesting Parts page]]

	===Using the online QC resources===		===Using the online QC resources===
-	Here at Registry, we want everyone to take a look at the results of the quality control measures we've taken this year and previous years, in order to make an informed decision when choosing to use a part. We've made sure to update the online repository for the Spring 2012 distribution with our quality control results.	+	Here at Registry, we want everyone to take a look at the results of the [[Help:Quality_Control\|quality control measures]] we've taken this year and previous years, in order to make an informed decision when choosing to use a part. We've made sure to update the online repository for the Spring 2012 distribution with our quality control results.

	The best way to use our quality control information is to use it on a part by part basis. As you design your project, make sure to check every part that you're interested in for its QC data. After searching for a part in the registry and arriving at its main page, click on the '''Get This Part''' link which will take you to the section listing various quality control information for a samples of that part and its location within the registry.		The best way to use our quality control information is to use it on a part by part basis. As you design your project, make sure to check every part that you're interested in for its QC data. After searching for a part in the registry and arriving at its main page, click on the '''Get This Part''' link which will take you to the section listing various quality control information for a samples of that part and its location within the registry.
-
-	~~For analyzing the QC information please see [http://partsregistry.org/Help:Quality_Control].~~


Line 94:		Line 92:

	===Linearized Plasmid Backbones===		===Linearized Plasmid Backbones===
-	The 2012 Distribution Kit also includes a set of four linearized plasmid backbones: pSB1A3, pSB1C3, ~~pSB1K3~~, and ~~pSB1T3~~. These plasmid backbones have been prepared via PCR and purified. Prior to ligation the linearized backbones will need to be digested with with EcoRI, PstI and DpnI, leaving two ends ready to be ligated to a Biobrick™ part. All 2012 submissions will need to be in pSB1C3, so we recommend using our pSB1C3 linearized backbone for that purpose.	+	The 2012 Distribution Kit also includes a set of four linearized plasmid backbones: pSB1A3, pSB1C3, pSB1T3, and pSB1K3.m1. These plasmid backbones have been prepared via PCR and purified. Prior to ligation the linearized backbones will need to be digested with with EcoRI, PstI and DpnI, leaving two ends ready to be ligated to a Biobrick™ part. All 2012 submissions will need to be in pSB1C3, so we recommend using our pSB1C3 linearized backbone for that purpose.

	You can find more in depth instructions on how to use and make your own linearized plasmid backbones on the [[Help:Protocols/Linearized Plasmid Backbones \| protocol page]]. ''Note: The linearized plasmid backbones need to be cut by EcoRI and PstI restriction enzymes prior to use.''		You can find more in depth instructions on how to use and make your own linearized plasmid backbones on the [[Help:Protocols/Linearized Plasmid Backbones \| protocol page]]. ''Note: The linearized plasmid backbones need to be cut by EcoRI and PstI restriction enzymes prior to use.''

Help:Spring 2012 DNA distribution

2012-05-14T13:42:14Z

Redirecting to Help:Distribution Kits

←Older revision		Revision as of 13:42, 14 May 2012
Line 1:		Line 1:
-	The 2012 Distribution contains over 1000 part samples as dried (miniprepped) DNA, with each sample [[Help:Quality_Control\|QC tested]] through sequencing, AB test plates, and restriction digests. While there is not enough DNA for assembly, you will be able to transform the DNA into cells and then make your own glycerol stocks of any part that you wish. This year's distribution does not contain all of the part samples available in the Registry Repository, but it does include both high-quality part samples from the past as well as some of the 2012 team favorites and highly requested parts during the 2011 season.	+	#redirect [[Help:Distribution_Kits]]
-		+
-	~~==What's included in the 2012 Distribution==~~	+
-	~~[[Image:2011-Distribution-Contents.jpg‎\|500px\|center]]~~	+
-		+
-	~~Your Spring 2012 Distribution contains the following:~~	+
-	* (5) 384w DNA Distribution Kit Plates	+
-	* Linearized Plasmid Backbones: pSB1C3	+
-	* Sponsor Information	+
-	* iGEM Stickers and Pins!	+
-		+
-	~~If there is an issue with your distribution kit, please send us an email to ''hq (at) igem . org''~~	+
-		+
-	~~==Getting Started==~~	+
-	If you're new to the Registry, iGEM, or synthetic biology, you'll want to read through our Help page before you get started. The [[Help:Learn\|Learn section]] is the proper place to start, and will take you through the basics.	+
-		+
-	~~Otherwise, read on!~~	+
-		+
-	~~===Locating a Part in the Distribution===~~	+
-	Before using the DNA plates, you should search the Registry for useful parts, which will also tell you if it has an available sample, its location (if they're in your 2012 Distribution), requirements, quality control (if they're correct), etc.	+
-		+
-	~~====Searching====~~	+
-	*The '''[[Help:Locating_Parts\|Catalog of Parts and Devices]]''' will allow you to browse parts and devices by various criteria, including function, chassis, standard, etc.	+
-	*The '''[[Help:Locating_Parts\|Search Tools]]''' will let you search by text or part name <more>.	+
-	*It is also possible to see the contents of the Kit Plates in their entirety. You can look at the [http://partsregistry.org/cgi/assembly/libraries.cgi DNA Repositories] section on the main page.	+
-	**Click on Spring 2012 Distribution to see the contents of all of your kit plates.	+
-	**Click on the 2012 Kit Plate of your choice, which will list all parts by their part name (BBa_..) in a plate along with their quality control information. Or you can click on the small part diagram below each Kit Plate link: "See a summary of the parts in this plate."	+
-	**These options will show you what is in each well of your plate, however they are not the best way to find specific parts you would like to use.	+
-		+
-	~~====Part Main Page====~~	+
-	~~When you find a part you can learn all about it by going through its [[Help:Nav/Part_Main_Page\|main page]] and sub-pages.~~	+
-		+
-	~~===Availability===~~	+
-	If you find a part that you would like to use, you need to make sure that a sample of the part is actually available. There are many parts in the Registry that people are still working on, or decided not to continue working on anymore, therefore we never received or do not yet have the physical DNA for them. This of course means that the DNA is not available in the Spring 2012 DNA Distribution. The simplest way to tell whether the part has an available sample is to look at the top right of the part's Main Page. If the part has an available sample the top part of the box will be green and say "DNA available."	+
-		+
-	~~===Ordering a part===~~	+
-	We've taken care to create a very well-rounded DNA Distribution this spring, however, should you find the part, or parts, that you require are not available in the distribution but available in the Registry, feel free to send us an email (hq [AT] igem [DOT] org) in order to request a sample. Just include the part name, the plasmid it's located in, and the source, and we'll send it out to you. For more information please see the [[Help:Requesting_Parts\|Requesting Parts page]]	+
-		+
-	~~===Using the online QC resources===~~	+
-	Here at Registry, we want everyone to take a look at the results of the quality control measures we've taken this year and previous years, in order to make an informed decision when choosing to use a part. We've made sure to update the online repository for the Spring 2012 distribution with our quality control results.	+
-		+
-	The best way to use our quality control information is to use it on a part by part basis. As you design your project, make sure to check every part that you're interested in for its QC data. After searching for a part in the registry and arriving at its main page, click on the '''Get This Part''' link which will take you to the section listing various quality control information for a samples of that part and its location within the registry.	+
-		+
-	~~For analyzing the QC information please see [http://partsregistry.org/Help:Quality_Control].~~	+
-		+
-		+
-	~~==Using the Spring 2012 DNA Distribution==~~	+
-	~~===Storage===~~	+
-	Since the distribution kit plates are comprised of dried DNA, they are quite stable for storage at room temperature. However once the DNA is resuspended in any of the wells, we recommend either storing the kit plate with its plastic cover in a -20C freezer, or aspirating the rest of the resuspended DNA from the well and keeping it separately in a -20C freezer.	+
-		+
-	~~The linearized plasmid backbones (25ng/ul at 50ul) should be stored at 4C or lower.~~	+
-		+
-	~~===DNA Kit Plate Orientation===~~	+
-	~~[[Image:IGEM06DistPlateTop.jpg\|right\|thumb\|200px\|Top view of plates containing dry DNA; <font color="red">red</font> circle indicates well 13H]]~~	+
-		+
-	The foil covers on each 384 well kit plate can be easily punched through with a pipette tip into the well of your choice. Unfortunately, the foil cover will also obscure both column and well markings. You can still find your part by correctly orienting the plate using the two notched corners as markers: well '''A1''' is located at the upper left corner of the plate when the long side of the plate with the notched corners is considered the bottom.	+
-		+
-	~~Once you know the location of your part, you will want to count across the plate starting with Column 1 until you get to Column 13 and down the plate starting with Row A until you get to Row H.~~	+
-		+
-	~~''Make sure that the two notched corners of the plate are oriented at the BOTTOM of the plate (see the "top view" image on the right for correct orientation)''~~	+
-		+
-	~~===DNA Kit Plate Instructions===~~	+
-	~~To use the DNA in the Distribution Kit you may follow these instructions:~~	+
-	:# With a pipette tip, punch a hole through the foil cover into the corresponding well of the Biobrick™-standard part that you want. [http://partsregistry.org/Help:Spring_2011_DNA_distribution#DNA_Kit_Plate_Orientation Make sure you have properly oriented the plate]. We recommend that you do not remove the foil cover, as it could lead to cross contamination between the wells.	+
-	:# Pipette 10uL of dH2O (distilled water) into the well. Pipette up and down a few times and let sit for 5 minutes to make sure the dried DNA is fully resuspended. We recommend that you do not use TE to resuspend the dried DNA.	+
-	:# [[Help:~~Transformation_Protocol\|Transform~~]] ~~1 or 2uL of the resuspended DNA into your desired competent cells, plate your transformation with the appropriate antibiotic* and grow overnight.~~	+
-	~~:#Pick a single colony and inoculate broth (again, with the correct antibiotic) and grow for 16 hours.~~	+
-	:#Use the resulting culture to [http://openwetware.org/wiki/Miniprep/Kit-free_high-throughput_protocol miniprep] the DNA AND make your own glycerol stock (for further instruction on making a glycerol see [http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria this page]). We recommend using the miniprepped DNA to run QC tests, such as restriction digests and sequencing.	+
-		+
-	''* To know which antibiotics to use, look at the plasmid that the part is in. The [http://partsregistry.org/wiki/index.php/Help:Plasmids/Nomenclature naming scheme] for plasmids is specifically designed to indicate antibiotic resistance.''	+
-		+
-	~~''Note: There is not enough DNA in each well to perform anything but [[Help:Transformation_Protocol\|transformations]]''~~	+
-		+
-	~~===Linearized Plasmid Backbones===~~	+
-	The 2012 Distribution Kit also includes a set of four linearized plasmid backbones: pSB1A3, pSB1C3, pSB1K3, and pSB1T3. These plasmid backbones have been prepared via PCR and purified. Prior to ligation the linearized backbones will need to be digested with with EcoRI, PstI and DpnI, leaving two ends ready to be ligated to a Biobrick™ part. All 2012 submissions will need to be in pSB1C3, so we recommend using our pSB1C3 linearized backbone for that purpose.	+
-		+
-	You can find more in depth instructions on how to use and make your own linearized plasmid backbones on the [[Help:Protocols/Linearized Plasmid Backbones \| protocol page]]. ''Note: The linearized plasmid backbones need to be cut by EcoRI and PstI restriction enzymes prior to use.''	+

Help:Distribution Kits

2012-05-11T16:58:59Z

←Older revision		Revision as of 16:58, 11 May 2012
Line 21:		Line 21:
	</html>		</html>
	{{TOCright}}		{{TOCright}}
-	The 2012 Distribution contains over 1000 part samples as dried (miniprepped) DNA, with each sample [[Help:Quality_Control\|QC tested]] through sequencing, AB test plates, and restriction digests. While there is not enough DNA for assembly, you will be able to transform the DNA into cells and then make your own glycerol stocks of any part that you wish. This year's distribution does not contain all of the part samples available in the Registry Repository, but it does include both high-quality part samples from the past as well as some of the 2012 team favorites and highly requested parts during the 2011 season.	+	The 2012 Distribution contains over 1000 part samples as dried (miniprepped) DNA, with each sample [[Help:Quality_Control\|QC tested]] through [[Help:Quality_Control#Sequencing_Results\|sequencing]], [[Help:Quality_Control#Antibiotic_Test_Plates\|AB test plates]], and [[Help:Quality_Control#Gel_Results\|restriction digests]]. While there is not enough DNA for assembly, you will be able to [[Help:Distribution_Kits#DNA Kit Plate Instruction\|transform the DNA]] into cells and then make your own glycerol stocks of any part that you wish. This year's distribution does not contain all of the part samples available in the [[Help:Repository\|Registry's Repository]], but it does include both high-quality part samples from the past as well as some of the 2012 team favorites and highly requested parts during the 2011 season.

	==What's included in the 2012 Distribution==		==What's included in the 2012 Distribution==
Line 28:		Line 28:
	Your Spring 2012 Distribution contains the following:		Your Spring 2012 Distribution contains the following:
	* (5) 384w DNA Distribution Kit Plates		* (5) 384w DNA Distribution Kit Plates
-	* Linearized Plasmid Backbones: pSB1A3, pSB1C3, pSB1K3, pSB1T3	+	* Linearized Plasmid Backbones: pSB1A3, pSB1C3, pSB1K3.m1, pSB1T3
-	* Sponsor Information	+
	* iGEM Stickers and Pins!		* iGEM Stickers and Pins!

Line 40:		Line 39:

	===Locating a Part in the Distribution===		===Locating a Part in the Distribution===
-	Before using the DNA plates, you should search the Registry for useful parts, which will also tell you if it has an available sample, its location (if they're in your 2012 Distribution), requirements, quality control (if they're correct), etc.	+	Before using the DNA plates, you should [[Help:Locating_Parts\|search the Registry]] for useful parts, which will also tell you if it has an available sample, its location (if they're in your 2012 Distribution), requirements, quality control (if they're correct), etc.

	====Searching====		====Searching====

SBOBET - fast as well as attached on the internet

2012-05-10T16:56:06Z

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Help:Synthetic Biology

2012-05-10T15:10:52Z

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	__NOTOC__		__NOTOC__
	==What is Synthetic Biology==		==What is Synthetic Biology==
-	~~[[Image:Video-place-holder.png\|thumb\|right\|Video: Introducing Synthetic Biology]]~~
	<blockquote> ''"A Scientist discovers that which exists; an Engineer creates that which never was."'' <br> - Theodore von Karmen</blockquote>		<blockquote> ''"A Scientist discovers that which exists; an Engineer creates that which never was."'' <br> - Theodore von Karmen</blockquote>

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	==Why Synthetic Biology based on standard parts==		==Why Synthetic Biology based on standard parts==
-	~~#Harvest through nature~~
-	~~#Improve~~
-	~~#Design to purpose~~
-
	To design these new systems, synthetic biologists will look to natural biological systems to find functional units of DNA or synthesize new ones that do not naturally exist. These functional units are tested and characterized and may become components in a biological device or system. We refer to them as '''[[Help:Parts\|parts]]''': a sequence of DNA that encodes for a specific biological function.		To design these new systems, synthetic biologists will look to natural biological systems to find functional units of DNA or synthesize new ones that do not naturally exist. These functional units are tested and characterized and may become components in a biological device or system. We refer to them as '''[[Help:Parts\|parts]]''': a sequence of DNA that encodes for a specific biological function.

Help:Contents

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	<div id="splash-desc">		<div id="splash-desc">
-	New to iGEM and the Registry? You'll want to head over to the <a href="http://partsregistry.org/Help:Learn">Learn</a> section first to get an introduction on synthetic biology based on standardized parts and the philosophy of iGEM and the Registry. We welcome suggestions via email (<strong>hq (at) igem . org</strong>) or on <a href="http://partsregistry.org/forum/Forum_List?n=Registry">the forum</a>. You can also look through the old help pages if you couldn't find what you're looking for.	+	New to iGEM and the Registry? You'll want to head over to the <a href="http://partsregistry.org/Help:Learn">Learn</a> section first to get an introduction on synthetic biology based on standardized parts and the philosophy of iGEM and the Registry. We welcome suggestions via email (<strong>hq (at) igem . org</strong>) or on <a href="http://partsregistry.org/forum/Forum_List?n=Registry">the forum</a>. You can also look through the <a href="http://partsregistry.org/Help:Archive">old help pages</a> if you couldn't find what you're looking for.
	</div>		</div>
	</td></tr>		</td></tr>