Part:BBa_V1005

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(Difference between revisions)

Current revision

DB3.1

E. coli strain containing the gyrA462 mutation, conveying recessive resistance to the ccdB gene.
Strain created by and available for purchase through Invitrogen

Usage and Biology

useful for propagating plasmids containing the ccdB operon.
gyrA462 enables ccdB containing plasmid propagation
streptomycin resistant

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]

References

Bernard P, Gabant P, Bahassi EM, Couturier M, Université Libre de Bruxelles. Positive-selection vectors using the F plasmid ccdB killer gene." Gene 1994 Oct 11;148(1):71-4. pmid:7926841. Pubmed
Bernard P, Gabant P, Université Libre de Bruxelles. Cloning and/or sequencing vector US Patent Number 5,910,438, 1999. Google Patents
Bernard P, Gabant P, Université Libre de Bruxelles. Cloning and/or sequencing vector US Patent Number 6,180,407 B1, 2001. Google Patents
Bernard P, Gabant P, Université Libre de Bruxelles. Cloning and/or sequencing vector US Patent Number 7,176,029 B2, 2007. Google Patents
Bahassi, et al., Université Libre de Bruxelles. Interactions of CcdB with DNA gyrase. Inactivation of Gyra, poisoning of the gyrase-DNA complex, and the antidote action of CcdA., J. Biol. Chem. 1999 274: 10936-10944. pmid:10196173. Pubmed

Retrieved from "http://partsregistry.org/Part:BBa_V1005"

[edit]

@@ Line 15: / Line 15: @@
 <partinfo>BBa_V1005 SequenceAndFeatures</partinfo>
-==References==
+===References===
-*"Positive-selection vectors using the F plasmid <i>ccdB</i> killer gene." Bernard P, Gabant P, Bahassi EM, Couturier M. 1994 Gene. Oct 11;148(1):71-4. [http://www.ncbi.nlm.nih.gov/pubmed/7926841 Pubmed]
+*Bernard P, Gabant P, Bahassi EM, Couturier M, Université Libre de Bruxelles. <i>Positive-selection vectors using the F plasmid </i>ccdB<i> killer gene.</i>" Gene 1994 Oct 11;148(1):71-4. pmid:7926841. [http://www.ncbi.nlm.nih.gov/pubmed/7926841 Pubmed]
+*Bernard P, Gabant P, Université Libre de Bruxelles. <i>Cloning and/or sequencing vector</i> US Patent Number 5,910,438, 1999. [http://www.google.com/patents?vid=USPAT5910438 Google Patents]
+*Bernard P, Gabant P, Université Libre de Bruxelles. <i>Cloning and/or sequencing vector</i> US Patent Number 6,180,407 B1, 2001. [http://www.google.com/patents?vid=USPAT6180407 Google Patents]
+*Bernard P, Gabant P, Université Libre de Bruxelles. <i>Cloning and/or sequencing vector</i> US Patent Number 7,176,029 B2, 2007. [http://www.google.com/patents?vid=USPAT7176029 Google Patents]
+* Bahassi, et al., Université Libre de Bruxelles. <i>Interactions of CcdB with DNA gyrase. Inactivation of Gyra, poisoning of the gyrase-DNA complex, and the antidote action of CcdA.</i>, J. Biol. Chem. 1999 274: 10936-10944. pmid:10196173. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10196173&query_hl=2&itool=pubmed_ExternalLink Pubmed]
-*US Patent Number 5,910,438 "Cloning and/or sequencing vector" Bernard P, Gabant P, University Libre de Bruxelles, 1999. [http://www.google.com/patents?vid=USPAT5910438 Google Patents]
-*US Patent Number 6,180,407 B1 "Cloning and/or sequencing vector" Bernard P, Gabant P, Universit Libre de Bruxelles, 2001. [http://www.google.com/patents?vid=USPAT6180407 Google Patents]
-*US Patent Number 7,176,029 B2 "Cloning and/or sequencing vector"  Bernard P, Gabant P, Universit Libre de Bruxelles, 2007. [http://www.google.com/patents?vid=USPAT7176029 Google Patents]
-*"Interactions of CcdB with DNA gyrase. Inactivation of Gyra, poisoning of the gyrase-DNA complex, and the antidote action of CcdA." Bahassi, et al. 1999 J. Biol. Chem. 274: 10936-10944 [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10196173&query_hl=2&itool=pubmed_ExternalLink Pubmed]
 *[[Help:Plasmids]]
 *[[Part:BBa_P1010]]