Regulatory
p(tetR)
Part:BBa_R0040
Designed by June Rhee, Connie Tao, Ty Thomson, Louis Waldman Group: Registry (2003-01-31)
From partsregistry.org
TetR repressible promoter
Sequence for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, aTc.
Usage and Biology
Medium strength promoter. [jb, 5/24/04]
From the reference article:"In contrast to tetracycline, anhydrotetracycline is a particularly useful inducer. It binds Tet R with an ~35-fold higher binding constant and thus allows to operate at very low concentrations. At the same time, its antibiotic activity is ~100-fold lower and concentrations of <50 ng/ml as required for the full induction of P LtetO-1 have no effect on the growth of E.coli."
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//promoter
//regulation/negative
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//promoter
//regulation/negative
//rnap/prokaryote/ecoli/sigma70
Reviews
1 Registry Star
1 Registry StarExperience: Works
5
(77)
4
(21)
3
(44)
2
(13)
1
(5)
Twins
Parameters
BBa_I714072 Deleted
BBa_K188027 Planning
BBa_K315018 Available
BBa_K315019 Available
BBa_K315023 Available
BBa_K315025 Available
BBa_K315035 Available
BBa_K831006 Deleted
BBa_K188027 Planning
BBa_K315018 Available
BBa_K315019 Available
BBa_K315023 Available
BBa_K315025 Available
BBa_K315035 Available
BBa_K831006 Deleted
| biology | |
| control | aTc, tetracyline |
| direction | Forward |
| negative_regulators | 1 |
| o_h | |
| o_l | |
| positive_regulators |
