Regulatory
LacI

Part:BBa_R0010

Designed by:   Group: Antiquity   (2003-01-31)

promoter (lacI regulated)

This part is an inverting regulator sensitive to LacI and CAP.

It contains two protein binding sites. The first binds the CAP protein, which is generally present in E.coli and is asocciated with cell health and availability of glucose. The second binds LacI protein.


  • In the absence of LacI protein and CAP protein, this part promotes transcription.
  • In the presence of LacI protein and CAP protein, this part inhibits transcription.
  • LacI can be inhibited by IPTG.
  • LacI is coded by BBa_C0010

Intrinsic noise value: 0.0707 (compare with R0011: 0.0040; R0051: 0.0869). See William_and_Mary iGEM 2015

Usage and Biology

This is a direct copy of bases 0365739 through 0365540 of the E. coli K-12 MG1655 genome, Genbank NC_000913 in reverse complement form. It is the natural promoter for the LacZYA operon. It includes the tail end of the LacI gene coding region, but no promoter region for that partial gene.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage in Chromobacterium Violaceum

Team Tec-Monterrey 2016 tested the Promoter BBa_R0010 in C. Violaceum. We were unable to confirm whether or not C. Violaceum had the lac operon, BLAST analysis of its genome suggested that it did not have this regulating system; our characterization demonstrates that the lac promoter can be used as a constitutive promoter in this chassis. There was no significant difference in the Fluorescence of the group of C. Violaceum with IPTG or without it, but in E. Coli it showed normal induction. Therefore, we can confirm that this promoter is constitutive in C. Violaceum. T--Tec-Monterrey--violaceumgrafiicauno.jpg T--Tec-Monterrey--violaceumgrafiicados.jpg


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
//classic/regulatory/uncategorized
Parameters
biology
controlIPTG, lacI
directionForward
negative_regulators1
o_h
o_l
positive_regulators