Part:BBa_K245051

From partsregistry.org

GyrB-SG-SG-CutA1

This part is made of two domains – GyrB, a 24 kDa fragment of gyrase subunit B, and CutA1, a copper binding protein. It is one of the constructs whose assembly can be regulated. When an aminocoumarin antibiotic called coumermycin is added, GyrB domains dimerize. Because this is a combination of dimerization and trimerization domains, the resulting structure is a lattice with hexagonal pores. Another aminocoumarin antibiotic novobiocin inhibits this structure. This part is similar to GyrB-CutA1 (BBa_K245050) but has extended linker according to Standard 37.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 428
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 100
Illegal AgeI site found at 238

Retrieved from "http://partsregistry.org/Part:BBa_K245051"

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