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Coding

Part:BBa_K199125

Designed by Olivia Ho-Shing   Group: iGEM09_MoWestern_Davidson   (2010-03-16)

From partsregistry.org

(Difference between revisions)
Current revision (23:31, 16 March 2010) (edit) (undo)
 
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<partinfo>BBa_K199125 short</partinfo>
<partinfo>BBa_K199125 short</partinfo>
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Synthesizes 3OC6HSL, which binds to LuxR. No LVA tag.
Synthesizes 3OC6HSL, which binds to LuxR. No LVA tag.
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Directly downstream of the ATG is 21 base pairs translated into 7 amino acids not seen at the beginning of wild-type LuxI (C0161). These base pairs will be mutated from this "optimized wild-type" sequence to become an AsiSI restriction site, a 5 bp-spacer, and an AscI restriction site. These two restriction sites will allow for unique coding sequences to easily be swapped out of the gene. This "optimized wild-type" sequence acts as a positive control before adding in the restriction sites or additional coding sequences.
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Directly downstream of the ATG is 21 base pairs translated into 7 amino acids not seen at the beginning of wild-type LuxI (C0161). This "optimized wild-type" sequence will be mutated to add an AsiSI restriction site, a 5 bp-spacer, and an AscI restriction site. These two restriction sites will allow for unique coding sequences to easily be swapped out of the gene. This "optimized wild-type" sequence acts as a positive control before adding in the restriction sites or additional coding sequences.
<!-- Add more about the biology of this part here
<!-- Add more about the biology of this part here

Current revision

LuxI autoinducer synthetase optimized for E. coli

Synthesizes 3OC6HSL, which binds to LuxR. No LVA tag.

Directly downstream of the ATG is 21 base pairs translated into 7 amino acids not seen at the beginning of wild-type LuxI (C0161). This "optimized wild-type" sequence will be mutated to add an AsiSI restriction site, a 5 bp-spacer, and an AscI restriction site. These two restriction sites will allow for unique coding sequences to easily be swapped out of the gene. This "optimized wild-type" sequence acts as a positive control before adding in the restriction sites or additional coding sequences.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 244


Retrieved from "http://partsregistry.org/Part:BBa_K199125"
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n/aLuxI autoinducer synthetase optimized for E. coli