Coding

Part:BBa_K124017

Designed by: Neil J. Parikh   Group: iGEM08_Brown   (2008-10-27)


Bacteriophage Lysis Cassette S105, R, and Rz

The gene cassette consists of the S105 protein (Holin), R protein (Endolysin), and Rz protein. When paired with an inducible promoter, the cassette is activated by the inducer and begins to produce the proteins which lyse the cell.

This cassette contains the mutant S gene, which can be found individually as BBa_K124014. The S variation allows for faster lysis.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/lysis
//function/celldeath
Parameters
None