Regulatory
p(osmY)

Part:BBa_J45992

Designed by: Stephen Payne   Group: iGEM2006_MIT   (2006-07-11)

Full-length stationary phase osmY promoter

BBa_J45992 is a stationary phase promoter derived from the promoter that controls transcription of osmY in Escherichia coli Yim-1992, Yim-1994. BBa_J45992 is active in stationary phase and under high osmotic pressure conditions.

BBa J45992.png

Usage and Biology

Expression of osmY is dependent on σS in vivo Hengge-Aronis-1993. Schellhorn et al. previously demonstrated that an osmY::lacZ fusion generated the highest transcriptional signal in stationary phase as compared to nine other σS-dependent promoter-lacZ fusions Schellhorn-1998, Vijayakumar-2004. In addition, the osmY::lacZ fusion generated only a small transcriptional signal during exponential growth.

<biblio>

  1. Yim-1994 pmid=8282684
  2. Yim-1992 pmid=1317380
  3. Hengge-Aronis-1993 pmid=8416901
  4. Vijaykumar-2004 pmid=15576800
  5. Schellhorn-1998 pmid=9829938

</biblio>

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
s
//promoter
//regulation/constitutive
//rnap/prokaryote/ecoli/sigmaS
Parameters
negative_regulators
positive_regulators