Coding
Hin LVA

Part:BBa_J31001

Designed by: Erin Zwack, Sabriya Rosemond   Group: iGEM2006_Davidson   (2006-07-12)

DNA invertase Hin tagged with LVA

BBa_J31001 codes for Hin invertase (Salmonella typhimurium, Ames strain TA100) with an LVA degredation tag. The degredation tag is added to ensure no Hin is floating around once we turn off the Hin promoter, allowing tight control of Hin-mediated DNA inversion. Please read the part design page for a note about the BioBricks on this part.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination
//function/recombination/hin
Parameters
ec_numnone
keggstm:STM2772
proteinHin-LVA
swissproP03013