Translational_Unit
Part:BBa_J18939
Designed by Raik Gruenberg Group: Affiliates (2010-01-26)
From partsregistry.org
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_J18939 short</partinfo> | <partinfo>BBa_J18939 short</partinfo> | ||
| - | + | P07 expression cassette | |
from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: | from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: | ||
Current revision
FKBP ~ bla-frag2
P07 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture:
- T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop
Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts.
Expression
Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity.
Reference
- Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 456
Illegal AgeI site found at 732
[edit]
Categories
//cds
//proteindomain/binding
//standard/25
//proteindomain/binding
//standard/25
Reviews
Parameters
Unrated Part
Experience:
5
(77)
4
(21)
3
(44)
2
(13)
1
(5)
| None |
