DNA Planning
Experience: Works
Get This Part
Plasmid_Backbone

Part:BBa_J176122

Designed by Brady Laughlin   Group: Guests   (2011-10-21)

From partsregistry.org

pcDNA3.1+ puro

pcDNA3.1+ puro/ ΔpCMV, based on pcDNA3.1+; no promoter; amp, puro
Alias: MV2

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal XbaI site found at 305
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
    Illegal NotI site found at 292
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal BglII site found at 12
    Illegal XhoI site found at 299
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal XbaI site found at 305
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal XbaI site found at 305
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
    Illegal NgoMIV site found at 737
    Illegal NgoMIV site found at 2148


Retrieved from "http://partsregistry.org/Part:BBa_J176122"
[edit]
Categories
Reviews
Unrated Part
Experience: Works
5
(77)
4
(21)
3
(44)
2
(13)
1
(5)
Parameters
n/apcDNA3.1+ puro