Part:BBa_C0061

From partsregistry.org

autoinducer synthetase for AHL

Synthesizes 3OC₆HSL, which binds to LuxR.

The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.

Usage and Biology

Enzyme for creating acyl-homoserine lactones from normal cell metabolites. All LuxI proteins direct the synthesis of specific acyl-HSLs and show sequence similarity.

Sequence and Features

The sequence of this part is normally followed by a barcode (Learn more about barcodes...)

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 637
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]

direction	Forward
ec_num	none
kegg	none
protein	luxI-LVA
swisspro	P12747
tag	LVA
uniprot	P48445