Plasmid_Backbone
pSB1A7
Part:pSB1A7
Designed by Karmella Haynes Group: iGEM2006_Davidson (2006-10-19)
From partsregistry.org
Transcriptionally insulated high copy BioBrick plasmid
pSB1A7 was constructed by adding double terminators (BBa_B0015) before the BioBrick prefix and after the BioBrick suffix in pSB1A3. The added terminators block read-through transcription coming from the vector backbone into the BioBrick MCS.
pSB1A7 was promoted from BBa_J31009 by Randy on 11/16/2006.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 2411
Plasmid lacks a suffix.
Illegal XbaI site found at 2426
Illegal SpeI site found at 1
Illegal PstI site found at 15 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2411 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2411
Illegal XbaI site found at 2426
Illegal SpeI site found at 1
Illegal PstI site found at 15
[edit]
Categories
//igem2006/davidson/favorite
//plasmidbackbone/assembly
//plasmidbackbone/copynumber/high
//standard/10
//plasmidbackbone/assembly
//plasmidbackbone/copynumber/high
//standard/10
Reviews
Parameters
Unrated Part
Group Favorite
Experience: Works
5
(77)
4
(21)
3
(44)
2
(13)
1
(5)
| chassis | |
| copies | 100-300 |
| mcs | BioBrick |
| origin | pMB1 |
| resistance | A |
