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Intermediate

Part:BBa_S03533

Designed by Davidson and Missouri Western   Group: iGEM2006_Davidson   (2006-08-02)

From partsregistry.org

pBad-HixC-TetB-RBSrev

Construction intermediate


Usage and Biology

Interestingly, this intermediate conveys Tetracycline resistance to E. coli. Note there is no promoter other than what the vector brings with it. This indicates a "read through" transcription. We are using this intermediate part as a way to screen for ways to insulate the BioBrick parts from "read through" transcription.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 505


Retrieved from "http://partsregistry.org/Part:BBa_S03533"
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