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Regulatory
lux pR

Part:BBa_R0062

Designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr   Group: Registry   (2003-01-31)

From partsregistry.org

Promoter (luxR & HSL regulated -- lux pR)

Promoter activated by LuxR in concert with HSL

The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.

This Plux promoter "pointing to the right" is the same sequence, but inverted, as part BBa_K199052.


Usage and Biology

Pretty good off in the absence of LuxR/HSL. [jb, 5/24/04]

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]


Retrieved from "http://partsregistry.org/Part:BBa_R0062"
[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//function/cellsignalling/LuxR
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Reviews
1 Registry Star
Experience: None
5
(77)
4
(21)
3
(44)
2
(13)
1
(5)
Twins
BBa_J69509 Planning
BBa_K332024 Deleted
Parameters
controlluxR, HSL
directionForward
negative_regulators
o_h
o_l
positive_regulators1