Part:BBa_P1010
From partsregistry.org
ccdB cell death gene
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The CcdB protein, constitutively expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant. |
P1010 is used when putting BioBrick parts into BioBrick plasmids. The part to be inserted and the plasmid are cut with BioBrick enzymes and mixed. The mixture will include both the original uncut or religated plasmid and the desired structure. However, because of CcdB, all of the cells containing the original plasmid die and the surviving colonies are the desired result.
Also see P1016, a smaller part containing ccdB but not the inactive ccdA contained in P1010.
BioBrick Construction Plasmids Containing This Part
Most BioBrick plasmids are delivered with the P1010 insert
see P1010 Physical DNA for the current list of plasmids that are available in this form. This particular Biobrick with its Plasmid backbone is considered a
Construction Plasmid.
Usage and Biology
For more information on how to use this brick, visit its Featured Parts:Cell Death page
References
- "Positive-selection vectors using the F plasmid ccdB killer gene." Bernard P, Gabant P, Bahassi EM, Couturier M. 1994 Gene. Oct 11;148(1):71-4. Pubmed
- US Patent Number 5,910,438 "Cloning and/or sequencing vector" Bernard P, Gabant P, University Libre de Bruxelles, 1999. Google Patents
- US Patent Number 6,180,407 B1 "Cloning and/or sequencing vector" Bernard P, Gabant P, Universit Libre de Bruxelles, 2001. Google Patents
- US Patent Number 7,176,029 B2 "Cloning and/or sequencing vector" Bernard P, Gabant P, Universit Libre de Bruxelles, 2007. Google Patents
- "Interactions of CcdB with DNA gyrase. Inactivation of Gyra, poisoning of the gyrase-DNA complex, and the antidote action of CcdA." Bahassi, et al. 1999 J. Biol. Chem. 274: 10936-10944 Pubmed
- Help:Plasmids
//plasmidbackbone/component/defaultinsert
| o_h | |
| protein | CcdB |
| rbs | |
| tag | None |
