DNA Available
Experience: Works
Get This Part
Coding

Part:BBa_K322237

Designed by Chris French and William Galdric Rostain   Group: Guests   (2010-09-26)

From partsregistry.org

Firefly luciferase from Photinus pyralis In luminescent reactions, light is produced by the oxidation of a luciferin: luciferin + O2 → oxyluciferin + light

This biobrick was made from BBa_I712019. 6 bases upstream of the start codon, present in BBa_I712019 (presumably to make a proper eukaryotic context for the start codon), were removed for improved expression in E. coli.

Note that the luciferase is temperature sensitive. Growing the host at 30°C will result in considerably increased brightness.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]


Retrieved from "http://partsregistry.org/Part:BBa_K322237"
[edit]
Categories
Reviews
Unrated Part
Group Favorite
Experience: Works
5
(77)
4
(21)
3
(44)
2
(13)
1
(5)
Parameters
emission555 nm