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Composite

Part:BBa_K273015

Designed by Karl Brune   Group: iGEM09_Uppsala-Sweden   (2009-10-17)

From partsregistry.org


PLlac - RBS - pdc - RBS - adh2(Y) - Ter

This construct encodes for an ethanol producing pathway. The pathway uses pyruvate as a precursor.

We used this construct for testing and characterization purposes in E. coli.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1096
    Illegal AgeI site found at 1243
    Illegal AgeI site found at 1621


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