This RpoA gene from Agrobacterium tumefaciens encodes the alfa subunit of the RNA Polymerase. This is necessary in E. coli so that the Vir box can be transcribed properly, allowing a functional VirA/VirG signaling system.
RpoA has illegal EcoRI restriction sites in its original sequence. We used site directed mutagenesis to get these sites out. EcoRI was mutated from GAATTC to GAATTT
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 978
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 951