Part:BBa_K238008

From partsregistry.org

VirA

This is VirA, a histidine kinase sensor originally found in Agrobacterium tumefaciens, Ti plasmid. It is part of the two-component VirA/VirG signal transduction system. This system is able to sense phenolic compounds and regulate gene transcription correspondingly.

VirA has illegal PstI restriction sites in its sequence. We used site directed mutagenesis to get these sites out. PstI was mutated from CTGCAG to CTGCAA.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 502
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 666
Illegal BglII site found at 1559
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1405