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Part:BBa_K191009

Designed by Le Thanh Tu NGUYEN   Group: iGEM09_EPF-Lausanne   (2009-10-19)

From partsregistry.org

LOVTAP (ILE427PHE)

Mutated version of BBa_K191006 : the amino acid #427 ILE was mutated into PHE.

Usage and Biology

The reasons and choix of this mutation are given by the results of modeling, under Mutations' section.

The mutated version of LovTAP should react faster and the conformational change should be more stable ( the protein stays in the active form for longer).

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 141
    Illegal PstI site found at 398


Retrieved from "http://partsregistry.org/Part:BBa_K191009"
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