Part:BBa_K191008

From partsregistry.org

Promoter LacI - RBS - LovTAP(ILE427PHE) - Term

Mutated version of BBa_K191003 : the amino acid #427 ILE was mutated into PHE.

Usage and Biology

The reasons and choix of this mutation are given by the results of modeling, under Mutations' section.

The mutated version of LovTAP should react faster and the conformational change should be more stable ( the protein stays in the active form for longer).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 238
Illegal suffix found in sequence at 1313
Illegal PstI site found at 629
Illegal PstI site found at 886
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 238
Illegal SpeI site found at 1314
Illegal PstI site found at 629
Illegal PstI site found at 886
Illegal PstI site found at 1328
Illegal NotI site found at 244
Illegal NotI site found at 1321
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 238
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 238
Illegal suffix found in sequence at 1314
Illegal PstI site found at 629
Illegal PstI site found at 886
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 238
Illegal XbaI site found at 253
Illegal SpeI site found at 1314
Illegal PstI site found at 629
Illegal PstI site found at 886
Illegal PstI site found at 1328

Retrieved from "http://partsregistry.org/Part:BBa_K191008"

[edit]

input_s	IPTG
positive_regulators
protein_out	LovTAP