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Regulatory
TrpR

Part:BBa_K191007

Designed by Le Thanh Tu NGUYEN   Group: iGEM09_EPF-Lausanne   (2009-10-16)

From partsregistry.org

Trp promoter

Promoter of Tryptophan operon ( Tryptophan operon is an operon that codes for the components of tryptophan's synthesis)

Usage and Biology

Presence of Tryptophan inhibits the expression of genes downstream of the regulator. As the hybrid protein LovTAP ( BBa_K191006 ) contains the corresponding regulatory domain, LovTAP can also bind to the regulator and and interferes with downstream gene's expression.


Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51


Retrieved from "http://partsregistry.org/Part:BBa_K191007"
[edit]
Categories
//regulation/negative
Reviews
Unrated Part
Experience: None
5
(77)
4
(21)
3
(44)
2
(13)
1
(5)
Parameters
input_sTrp, LovTAP
negative_regulators