DNA Sent
Experience: None
Get This Part
Coding
LovTAP

Part:BBa_K191006

Designed by Le Thanh Tu NGUYEN   Group: iGEM09_EPF-Lausanne   (2009-10-16)

From partsregistry.org

LOVTAP

Part BBa_K191006 contains coding sequence of the hybrid protein LovTAP. This hybrid protein has a light-sensitive domain (Lov means : light, oxygen, voltage) and a regulatory domain (part of the Trp repressor). The fused protein allows transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain, therfore results in an increase or decrease of the regulatory domain's affinity for its DNA binding site. The activation of the LovTAP protein (binding to DNA) is achieved by illumination @ 470 nm

For more details, please consult the section of LovTAP system on team EPF-Lausanne wiki.

For more details, please consult the section of Introduction LovTAP on team EPF-Lausanne wiki

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 141
    Illegal PstI site found at 398


[edit]
Categories
//function/sensor/light
Reviews
Unrated Part
Group Favorite
Experience: None
5
(77)
4
(21)
3
(44)
2
(13)
1
(5)
Parameters
None