Part:BBa_K191003
From partsregistry.org
Promoter Lac I - RBS - LovTAP - Term
Part BBa_K191003 codes for the hybrid protein LovTAP. This hybrid protein has a light-sensitive domain (Lov means : light, oxygen, voltage) and a regulatory domain (part of the Trp repressor). The fused protein allows transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain, therfore resulting in an increase or decrease of the regulatory domain's affinity for its DNA binding site. The activation of the LovTAP protein (binding to DNA) is achieved by illumination @ 470 nm
For more details, please consult the section of LovTAP system on team EPF-Lausanne wiki.
Usage and Biology
In order to be able to test the efficiency of the fused protein LovTAP, its expression is induced by IPTG (thanks to the LacI promoter). Two other parts were also constructed as the read out systems of LovTAP : BBa_K191004 and BBa_K191005
All three parts together give the following system, where LovTAP activities can be measured through RFP fluorescence intensity (both with with static and kinetic measurements)
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 365
Illegal PstI site found at 622 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 365
Illegal PstI site found at 622 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 365
Illegal PstI site found at 622 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 365
Illegal PstI site found at 622
| input_s | IPTG |
| positive_regulators | |
| protein_out | LovTAP |
