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Intermediate

Part:BBa_K182001

Designed by Daniel J. Leirer, Md. Risat ul Haque   Group: iGEM09_Aberdeen_Scotland   (2009-07-06)

From partsregistry.org


Intermediate Part consisting of LacI ( RBS+, Term +) and a Tet operator.

This is an intermediate part using the biobrick BBa_I732820 (RBS, LacI, Term) upstream and the Biobrick BBa_R0040 (TetR repressible promoter) downstream.

It comes with a ribosome binding site upstream of LacI and a Terminator downstream (BBa_B0015) There is no Promoter upstream of LacI, meaning that LacI will not be expressed in this piece.

The TetR repressible promoter BBa_R0040 is downstream of the Terminator.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]


Retrieved from "http://partsregistry.org/Part:BBa_K182001"
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