Part:pSB1AK8

From partsregistry.org

(Redirected from Part:BBa J52017)

Eukaryotic terminator vector (formerly BBa_J52017)

This vector is BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

RS 19:58, 24 November 2008 (UTC): The original entry of this part included both a plasmid and plasmid backbone. It has been split into a plasmid backbone and part. The physical Registry distribution has been updated accordingly.

The original part is now pSB1AK8-BBa_P1010.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal XhoI site found at 1272
Illegal XhoI site found at 2298
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 3405
Illegal suffix found at 2
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal XbaI site found at 3420
Illegal SpeI site found at 2
Illegal PstI site found at 16

Retrieved from "http://partsregistry.org/Part:pSB1AK8"

[edit]

chassis
copies
mcs	BioBrick
origin	pMB1
resistance	AK