Plasmid_Backbone
pSB1AK8
Part:pSB1AK8
Designed by Monika Ciglic Group: iGEM2006_Slovenia (2006-10-14)
From partsregistry.org
(Redirected from Part:BBa J52017)
Eukaryotic terminator vector (formerly BBa_J52017)
This vector is BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
- RS 03:20, 9 December 2009 (UTC): This plasmid backbone is not fully compliant with Assembly standard 10. It is missing a C in the BioBrick suffix. The NotI and PstI sites overlap as the sequence is currently specified in the Registry. It is not known whether this error is simply a user entry error. Or if the deletion is present in the physical DNA as well.
- RS 19:58, 24 November 2008 (UTC): The original entry of this part included both a plasmid and plasmid backbone. It has been split into a plasmid backbone and part. The physical Registry distribution has been updated accordingly.
The original part is now pSB1AK8-BBa_P1010.
Sequence and Features Content-type: text/html
Software error:
Can't call method "sequence" without a package or object reference at /web-site/partsregistry.org/cgi/lib/AssemblySystem.pm line 202.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
[edit]
Categories
//chassis/eukaryote
//igem2006/slovenia/favorite
//plasmidbackbone/operation
//standard/10
//igem2006/slovenia/favorite
//plasmidbackbone/operation
//standard/10
Reviews
1 Registry Star
Parameters
1 Registry StarGroup Favorite
Experience: Works
5
(77)
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2
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1
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| chassis | |
| copies | |
| mcs | BioBrick |
| origin | pMB1 |
| resistance | AK |

