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Part:BBa_J45250

Designed by André Green II   Group: iGEM2006_MIT   (2006-10-28)

From partsregistry.org

Stationary-phase-dependent banana odor generator

The stationary phase dependent banana odor generator (BBa_J45250) catalyzes the conversion of the precursor isoamyl alcohol to the odor isoamyl acetate that has a banana smell. The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (BBa_J45992) and an odor enzyme generator (BBa_J45199). Odor enzyme generators take as input a transcriptional signal and produce as output an enzyme that catalyzes production of an odor from a chemical precursor.

Usage and Biology

  • Supplement cultures with 5 mM isoamyl alcohol to produce banana odor.
  • See BBa_J45014 for details.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 327
    Illegal BamHI site found at 1654
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]


Retrieved from "http://partsregistry.org/Part:BBa_J45250"
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Categories
//classic/generator/prct
//function/odor
//iGEM2006/MIT/favorites
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Parameters
o_h
proteinATF1
rbs147
tagNone