Coding
BAT2
Part:BBa_J45008
Designed by Veena Venkatachalam Group: iGEM2006_MIT (2006-06-07)
From partsregistry.org
branched-chain amino acid transaminase (BAT2); converts leucine to alpha-ketoisocaproate
BBa_J45008 encodes a branched-chain amino acid transaminase derived from the BAT2 gene from Saccharomyces cerevisiae. BAT2 catalyzes the first step in isoamyl alcohol biosynthesis: conversion of leucine to α-ketoisocaproate via enzymatic reaction 2.6.1.42.
Usage and Biology
α-ketoisocaproate can be converted to 3-methylbutanal and then isoamyl alcohol which is a precursor to isoamyl acetate (banana odor).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1030
[edit]
Categories
//function/biosynthesis/odorant
//function/odor
//igem2006/mit/favorites
//function/odor
//igem2006/mit/favorites
Reviews
Parameters
Unrated Part
Group Favorite
Experience: Fails
5
(77)
4
(21)
3
(44)
2
(13)
1
(5)
| ec_num | 2.6.1.42 |
| kegg | sce:YJR148W |
| protein | BAT2 |
| swisspro | P47176 |

