Part:BBa_J45004

From partsregistry.org

SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali

BBa_J45004 encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I derived from BSMT1 from Petunia x hybrida. BSMT1 catalyzes the conversion of benzoic acid to methyl benzoate and salicylic acid to methyl salicylate. Methyl benzoate has a floral smell and methyl salicylate has a wintergreen smell.

Usage and Biology

Substrate	pH optimum	Apparent K_m for Substrate (μM)	k_cat × 10^-2 (s^-1))	k_cat/K_m (mM^-1⋅s^-1))
SAM_SA		1.93 ± 0.18	1.45 ± 0.024	7.65 ± 0.618
SAM_BA		15.78 ± 1.34	0.75 ± 0.023	0.48 ± 0.047
Salicylic acid	7.0 to 8.0	51.55 ± 2.73	1.2 ± 0.16	0.23 ± 0.038
Benzoic acid	7.5	1273.2 ± 37	0.39 ± 0.04	0.003 ± 0.0003

Negre F, Kish CM, Boatright J, Underwood B, Shibuya K, Wagner C, Clark DG, and Dudareva N. Regulation of methylbenzoate emission after pollination in snapdragon and petunia flowers. Plant Cell 2003 Dec; 15(12) 2992-3006. doi:10.1105/tpc.016766 pmid:14630969. PubMed HubMed [Negre-2003]

All Medline abstracts: PubMed HubMed

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 663
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]

Retrieved from "http://partsregistry.org/Part:BBa_J45004"

[edit]

ec_num	none
kegg	none
protein	BSMT1
swisspro	Q84UB5