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Designed by Tom Hinson   Group: iGEM2006_Imperial   (2006-07-17)

From partsregistry.org

Prey Molecule Generator (AHL) + GFP Reporter

Prey Genotype + Reporter

Usage and Biology

Biology:

• In nature, quorum sensing is used for cell-cell communication to co-ordinate particular behaviours or actions once the cell population has reached a sufficiently large size.
• This part generates the quorum sensing molecule, AHL via a positive feedback loop:

• The tetR promoter constitutively produces LuxR. AHL then forms a complex with luxR and lux pR promoter, expressing LuxI and GFP. LuxI causes the production of AHL. GFP can be used to monitor transcription of LuxI as a check for the functionality of the part.

• The positive feedback loop is triggered once cell population reaches a certain size.
• The behaviour of this part was successfully modelled, generating a transfer curve of AHL vs. Time.

Usage:

• This part was created by iGEM@Imperial 2006 to be used as the prey part in their 2006 project 'Engineering a Molecular Predation Oscillator' to generate a the production of Acyl-Homoserine Lactone (a quorum sensing molecule).

Other Uses:

• This part could be used to amplify a signal: (see experience page)

• A small amount of AHL present in the cell can be used to trigger the production of much more AHL via the positive feedback loop of this part.

• This part could be used to monitor cell population size.

Sequence and Features

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