Generator
Part:BBa_J3106
Designed by Davidson and Missouri Western Group: iGEM2006_Davidson (2006-08-02)
From partsregistry.org
pBad-HixC-TetB-RBSrev-HixC-TT-RE
This part contains the tetracycline resistance in the backwards orientation as well as a backwards RBS. These parts are flanked by HixC sites so when this part is placed downstream of the Hin or HinLVA cassettes the RBS and the tetracycline resistance will be in the correct orientation and the cell will express tetracyline resistance.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 505
[edit]
Categories
//function/recombination/hin
Reviews
Parameters
Unrated Part
Experience: Issues
5
(77)
4
(21)
3
(44)
2
(13)
1
(5)
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