Part:BBa_J3103

From partsregistry.org

pBad-HixC-RBS-TetF-HixC-TT-RE

This part contains the coding region for tetracylcine resistance flanked by Hix C sites. This part is going to be used to test the optimal location of the RBS. If the Hin(BBa_J31000) or Hin LVA (BBa_J31001) genes are placed upstream of this part there is a possibility that the coding region between the Hix C sites is inverted.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
Illegal BamHI site found at 483
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 509
Illegal NgoMIV site found at 877
Illegal NgoMIV site found at 1037

Retrieved from "http://partsregistry.org/Part:BBa_J3103"

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