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Part:BBa_J31012

Designed by Karmella Haynes   Group: iGEM2006_Davidson   (2006-11-10)

From partsregistry.org


Tet resistance expression cassette

Double terminator BBa_B0015 (TT) placed upstream of pBad, RBS and the TetR coding region. TT is placed upstream of the promoter to block read-through transcription. This cassette will be inserted into various plasmids of different copy numbers to gauge cell survival on increasing concentrations of tetracycline vs. the number of Tet+ plasmids in a cell.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 236
    Illegal BamHI site found at 654
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 680
    Illegal NgoMIV site found at 1048
    Illegal NgoMIV site found at 1208


Retrieved from "http://partsregistry.org/Part:BBa_J31012"
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