DNA Available
1 Registry Star
Get This Part
Coding
tetA(C)f

Part:BBa_J31007

Designed by Sabriya Rosemond, Erin Zwack   Group: iGEM2006_Davidson   (2006-07-12)

From partsregistry.org

tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]

BBa_J31007 is the coding region for tetracyline resistance [TetA(C) inner-membrane-associated protein] in the forward orientation. This part requires a promoter and RBS for proper expression.

Usage and Biology

Cells expressing BBa_J31007 are able to grow on media containing tetracycline; overexpression, however, is detrimental to cell growth.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 290
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 316
    Illegal NgoMIV site found at 684
    Illegal NgoMIV site found at 844


[edit]
Categories
//cds/selectionmarker/antibioticresistance
//igem2006/davidson/favorite
Reviews
1 Registry Star
Group Favorite
Experience: Works
5
(77)
4
(21)
3
(44)
2
(13)
1
(5)
Parameters
functionRep
ligandstetracycline
proteinTetA(C)
swissproP02981