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DNA Planning
Experience: Works
Part:BBa_J01008
Entered: 2005-11-05
Designed by Golden Bear Group: iGEM_Berkeley
From partsregistry.org
Riboregulator key 1
BioBricked version of Isaacs' riboregulator trans-activating key, taR12
Usage and Biology
The Isaacs key1 riboregulator taR12 is modeled on the hok/sok post-segregational killing method of plasmid R1. It makes 25 base pairs with its counterpart, the riboregulator J01010 (aka "lock 1" or "crR12").
Isaacs documentation (see reference #1):
Upon induction of the araC promoter (using 0.25% arabinose solution), the taR12 key activated the corresponding crR12 lock to express the downstream indicator, GFP, to levels of fluorescence 19-fold greater than without taR12 expression. Maximal expression was reached at 70 minutes after addition of arabinose solution, and was maintained after that point.
Sequence and Features
