Part:BBa_I742141

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sam5 (coumarate hydroxylase) coding sequence

This is the coding sequence of sam5, encoding 4-coumarate-3-hydroxylase from the bacterium Saccharothrix espanaensis. The enzyme converts p-coumaric acid to caffeic acid. Accession: ABC88666. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006). Note: contains a PstI site. This will be removed by site directed mutagenesis in due course but is still present, near the 3' end, in this version.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1420
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1420
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1420
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1420
Illegal NgoMIV site found at 21
Illegal NgoMIV site found at 29
Illegal NgoMIV site found at 779
Illegal NgoMIV site found at 952
Illegal NgoMIV site found at 1354
Illegal NgoMIV site found at 1358

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