Part:BBa_I714038

From partsregistry.org

pSC101 with relaxase and oriT deleted

This is a conjugative plasmid pSC101 with mobA (relaxase coding gene) and oriT (origin of transfer) knocked out, providing all other necessary proteins for conjugative transfer of plasmid pSC101 in the help of conjugative plasmid R751. The plasmid is a mutant, unable to perform conjugative functions, but when pSC101 MobA protein is supplied in the R751 strain, any plasmid with pSC101 oriT transfer origin in this chasis can be conjugatively transfered into recipient cells.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 4027
Illegal PstI site found at 2936
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 375
Illegal XhoI site found at 7013
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 4027
Illegal PstI site found at 2936
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 4027
Illegal PstI site found at 2936
Illegal NgoMIV site found at 401
Illegal NgoMIV site found at 769
Illegal NgoMIV site found at 929
Illegal NgoMIV site found at 1283
Illegal NgoMIV site found at 5479
Illegal NgoMIV site found at 7568
Illegal AgeI site found at 1687

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