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Composite

Part:BBa_I52000

Designed by Reshma Shetty   Group: Knight Lab, MIT   (2006-03-13)

From partsregistry.org

ccdB and minimal pUC19 derived high copy origin

This part is designed to be located in the multiple cloning site of low copy BioBrick vectors. BBa_P1011 provides selection against non-insert containing clones and BBa_I50020 enables the base vector to be propagated at high copy for ease of purification

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 443
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]


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