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Part:BBa_I50050

Designed by Reshma Shetty   Group: Knight Lab, MIT   (2006-02-03)

From partsregistry.org


R6K replication origin

R6K minimal origin of replication. Only contains gamma origin and requires pir+ strain for replication. Copy number is dependent on amount of pir protein in the cell.


Usage and Biology

See also EZ-Tn5† <R6K&#947;ori /KAN-2> Sequence at http://www.epibio.com/sequences/R6Kori_Kan2.asp and general information at http://www.frankwu.com/R6K.html.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]


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//plasmid/component/origin
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