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Experience: Fails
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pSC101

Part:BBa_I50040

Designed by Reshma Shetty   Group: Knight Lab, MIT   (2006-02-03)

From partsregistry.org

nonfunctional pSC101 origin of replication

BBa_I50040 was designed based on the pSC101 origin of replication with several mutations introduced to eliminate endonuclease recognition sites.

However, testing revealed it to be nonfunctional as a replication origin. BBa_I50042 is functional version of BBa_I50040.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]


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