Regulatory
Part:BBa_I13453
Designed by jkm Group: Endy Lab (2005-05-25)
From partsregistry.org
Pbad promoter
PBad promoter from I0500 without AraC.
Usage and Biology
Has been used as a second promoter in a system containing BBa_I0500 (PBad+AraC). In this system, it showed behavior qualitatively indistinguishable from the BBa_I0500 copy of PBad. Has not been tested independent of AraC. A second part, BBa_I13458, should allow decoupling of PBad and AraC.
See this OpenWetWare article on pBAD and lac promoters for additional usage and biology information
Characterization
For the 2009 iGEM competition, British_Columbia characterized BBa_I13453 in the context of a pBAD promoter family. For the results of this characterization, see here.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
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Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
//classic/regulatory/uncategorized
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
//classic/regulatory/uncategorized
Reviews
1 Registry Star
Parameters
1 Registry StarExperience: Works
5
(77)
4
(21)
3
(44)
2
(13)
1
(5)
| negative_regulators | |
| positive_regulators | 1 |

