Part:BBa_E0033

From partsregistry.org

LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)

To greatly reduce the number of bases, this is only a portion of the LacZ gene. It can be used as a detection system with N-terminal deletion mutants of lacZ. Combination of lacZ-alpha with such a deletion mutant protein restores enzyme activity that can be assayed colorimetrically or more sensitively with chemiluminescent substrates.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 93
Illegal XhoI site found at 144
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]

Retrieved from "http://partsregistry.org/Part:BBa_E0033"

[edit]

Categories

//cds/enzyme
//function/coliroid
//function/reporter
//function/reporter/color

Reviews

1 Registry Star

Experience:

(77)

(21)

(44)

(13)

(5)

Parameters

abs	varied
emission
emit
excitation
excite
lum	varied
protein	LacZ-alpha
tag	None