Coding
cI 434
Part:BBa_C0052
Designed by Maia Mahoney Group: Registry
From partsregistry.org
cI repressor from phage 434 (+LVA)
The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, Part:BBa_R0052. The sequence contains a LVA tag for faster degradation and has no RBS.
Usage and Biology
434 cI is a member of the lamboid cI protein family.
The coding sequence has been modified from wild type cI to contain an LVA tag and two TAA stop codons; its EcoRI recognition site has been mutated out by changing base 36 from T to C to be BioBrick-compatible.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
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Categories
//cds
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
Reviews
1 Registry Star
Parameters
1 Registry StarExperience: None
5
(77)
4
(21)
3
(44)
2
(13)
1
(5)
| direction | Forward |
| function | Rep |
| ligands | RecA? |
| protein | CI 434-LVA |
| swisspro | P16117 |
| tag | LVA |

