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Coding
cI 434

Part:BBa_C0052

Designed by Maia Mahoney   Group: Registry

From partsregistry.org

cI repressor from phage 434 (+LVA)

The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, Part:BBa_R0052. The sequence contains a LVA tag for faster degradation and has no RBS.


Usage and Biology

434 cI is a member of the lamboid cI protein family.

The coding sequence has been modified from wild type cI to contain an LVA tag and two TAA stop codons; its EcoRI recognition site has been mutated out by changing base 36 from T to C to be BioBrick-compatible.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]


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Categories
//cds
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
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Parameters
directionForward
functionRep
ligandsRecA?
proteinCI 434-LVA
swissproP16117
tagLVA