Part:BBa_B0044

From partsregistry.org

Topoisomerase I mediated cloning site (reverse)

Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning. This site belongs 3' of the cloning site. It is the reverse complement of BBa_B0043.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]

Retrieved from "http://partsregistry.org/Part:BBa_B0044"

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