Help:Sending Parts
From partsregistry.org
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Sending Parts |
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2012 Submission Details
All part samples must be submitted in pSB1C3
- The Registry only accepts part samples in pSB1C3, the Registry's plasmid backbone shipping standard. Why?.
- The 2012 DNA Distribution kit contains a sample of pSB1C3 linearized plasmid backbone for transferring your parts into pSB1C3.
- You can also transform a sample of pSB1C3 from the kit plates, make your own linearized plasmid backbone, or request more pSB1C3 linearized backbone by sending an email to hq [at] igem [dot] org.
- If you have issues with sending any of your part samples in pSB1C3 send us an email immediately!
"Quality over quantity" is our motto!
- Teams and labs should submit parts that are well documented and characterized.
- For examples of well documented parts check out BBa_K274210, BBa_F2620, and BBa_E0040
- Sending a few well-documented parts is always better than sending several that aren't!
Read the instructions and prepare your shipment!
- Be sure to complete your submission form in its entirety! You can follow the instructions below.
- Prepare your part samples according to the requirements of the submission format that you've chosen.
Provide tracking information for your DNA shipment.
- Tracking information is required to finalize your submission form, so be sure to use a carrier that will provide a tracking number for your DNA shipment.
- The tracking information will allow you (and the Registry) to keep track of the progress of your DNA shipment.
Starting a New DNA Submission
Before starting a submission make sure that all the parts you intend to send are documented on the Registry. See the "Adding a Part" tutorial or the video!
When you are ready to send your parts to the Registry, go to the DNA Submission main page. To begin the submission process click on “Start a New DNA Submission Now.”
Your Information
- Make sure that the username and full name that we have listed for you is correct.
- Use the drop down box to select your group, this will be the iGEM Team or Lab you are sending the parts on behalf of.
- Correct your email if necessary and enter a contact number as well.
Format
- You must tell us in what format you are sending your DNA.
- Be sure to follow the preparation requirements for the accepted formats.
- You should also let us know if there are any comments or special instructions that we must know about.
- Proceed to entering sample information by clicking on the button at the bottom of the page.
Sample Information
At the top of this page you will see your shipment #, which is what you'll use to identify and keep track of your shipment. The shipment # must be written clearly on your physical shipment.
- Enter the information for your first part, beginning with the part number.
- Remember, your part must be documented on the Registry before you can add it as a sample.
- You must enter the plasmid backbone (pSB1C3) that your part is in.
DO NOT...
- Leave the plasmid field empty!
- Write in an incorrect plasmid!
Once you have entered the part number and plasmid backbone for your part, the software will automatically fill in the antibiotic resistance based on the plasmid backbone that you have entered (the antibiotic abbreviation is the first letter of the antibiotic, C for pS1C3). Please check to make sure that the resistance is correct. You may change it manually if it is not correct.
Special Considerations
For each part you're sending, please check any of the boxes that apply to that part. If:
Toxic to E.coli strains
- We need to know specifically whether your part is toxic to the NEB 10b strain. We will be transforming all parts into NEB 10b and we need to know if this will be a problem for any of your parts.
Sending a plasmid backbone
- If you are sending us a new plasmid backbone, you need to check the appropriate box (see below for more plasmid information). BBa_J04450 is the standard shipping part (and required) for submitting plasmid backbones to the Registry.
It is not a part or plasmid
Contact HQ!
Team has sequenced this part
- Please let us know by checking the box if you or your team has sequenced this part. This is not a box to have your part sequenced. At the moment the Registry does not collect any sequence data or trace files, but you should include this information on your part's main page.
Finish Adding All Your Samples
Once you have finished adding all of the information for your part, click on the “save” link and then “add another sample” button if you are sending more parts to the Registry. The information for each sample is automatically saved to this shipment number once you click on the “save” link.
Shipment Information
When you are done filling in the information for all of your samples, click on the “I am finished specifying the samples” button.
Be prepared with your tracking #! Have all your details (Carrier, Tracking #, and Send Date) on hand as you enter the information. If you do not have this information ready, you can always come back to your submission form at a later time, but do not select "I am finished filling in the information, Send this batch to the Registry." Once you select "I am finished filling in the information, Send this batch to the Registry" you will no longer be able to edit your shipment as it will be finalized.
When you are finished providing shipping details, click on the “I am finished filling in this information” button to send your information to the Registry.
This finalizes your shipment information. You will see a confirmation page that congratulates you on submitting your information. It will also show all parts that are part of that shipment number. Print out two copies of this page: one for your records, and one to include with your shipment.
At this point you can go back to the DNA Submission Registry page and click on “see your DNA submissions” to check on the status of your shipment. You will be able to see when we have received your shipment.
Shipping Instructions
Make sure your team name, shipment #, and send date are written clearly on your submission!
Be sure to use a shipping carrier that that will let you track the shipment. For iGEM competition teams, physical DNA for parts is due by their Regional deadline for part submission and if we do not receive your shipment, we will track your package and give that information to the judges. If your shipment does not have tracking information and has not arrived at the Registry we will count your physical DNA as not available. You will not be able to receive a medal, as per the iGEM requirements.
*Important*: When shipping your samples, make sure to include a detailed description of the package so that it gets through customs. Write the following:
Dry/liquid DNA, non-hazardous, non-infectious, non-regulated. For research use only.
Mark Parts as Favorites
To mark your parts as your team's Favorites follow the instructions below.
- go to the part Main Page on partsregistry.org
- go to the Hard Information page
- click Edit on the right-hand side of the Group Favorite box
- change to Yes
Notes
- Accepting bacteria
- The Registry does not accept bacteria shipments at this time. Please only send isolated plasmid DNA. You may contact us if you have any questions regarding this policy.
- Editing your shipment after it has been finalized
- You may edit your shipment at any point up until the time you finalize your shipment after entering your shipping details. After you have finalized your shipment and sent all parts and information to the Registry, you will not be able to edit your online submission form. You will need to create a new submission for with the correct information and contact hq [at] igem [dot] org. Please include your original submission number, your name, your team name, and the new submission number.
- Part and plasmid specification details
- In specifying your parts you must clearly understand the relationship between parts and plasmids backbones. You must be explicitly aware that a part does NOT include the flanking restriction enzyme sites (called BioBrick™ prefix and suffix if you are using the BioBrick™ standard for which the restriction enzymes are: EcoRI, XbaI, SpeI, PstI). These sites are included in the plasmid backbone that your part is in (pSB1C3). Your part, then, begins with the first letter (A, C, T, G) following the prefix cloning site of the plasmid and ends with the last letter preceding the suffix cloning site of the plasmid.
- Take a look at this help page for more information on Biobrick™ parts and plasmids and how they are defined.
FAQ
My part is meant to operate in another plasmid backbone, do I still have to submit it in pSB1C3? Yes! pSB1C3 is the packaging for your part sample. It's expected that anyone using your part sample will use it outside of pSB1C3, and move it to an appropriate plasmid backbone. Since pSB1C3 is a BioBrick RFC[10] backbone, they can easily transfer it to another plasmid backbone for operational, measurement, assembly, or other purposes.
If there are reasons that would prevent your part from propagating or being maintained in pSB1C3, please send us an email.
Why do I have to submit my part samples in pSB1C3?
At iGEM HQ, submission in pSB1C3 allows us to analyze and maintain part samples properly and efficiently:
- pSB1C3 has VF2 and VR primer sites, which means all part samples can be sequenced using the VF2 and VR primers
- For restriction digests, plasmid backbone length remains the same, allowing for easier analysis of part length
- Using pSB1C3, ensures that all submissions will be chloramphenicol resistant
- pSB1C3 is a BioBrick RFC[10] backbone, so it will flank the part sample with the BioBrick prefix and suffix
- This means that all submitted parts will be BioBrick RFC[10] compatible, and will work with any current assembly method
Since all new parts submitted to the Registry will be in pSB1C3 (and as we transfer the older parts over), these same benefits are passed on to users: when you get part samples in pSB1C3 you can sequence them and run quality control tests using the same methods.
Have any questions? Contact hq [AT] igem [DOT] org.
