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  • ...L (1uM) through repeated 1:100 dilutions had no discernable impact on cell growth compared to negative controls (0uM), but had the unexpected effect of minim ...to find a concentration range where cell growth was neither substantially delayed nor was Beta-Galactosidase production only modestly increased. We started
    25 KB (3,932 words) - 03:45, 19 September 2015
  • ...p://parts.igem.org/Part:BBa_K238013">BBa_K238013</a></html>, expression is delayed and rises strongly after about four hours. This was tested in a Miller Assa ...ffered greatly, they were normalized to the "dark" sample of each point of time, which was set to 100 %.]]
    4 KB (616 words) - 10:40, 14 October 2011
  • ...L (1uM) through repeated 1:100 dilutions had no discernable impact on cell growth compared to negative controls (0uM), but had the unexpected effect of minim ...to find a concentration range where cell growth was neither substantially delayed nor was Beta-Galactosidase production only modestly increased. We started
    17 KB (2,873 words) - 03:44, 19 September 2015
  • ...tutive promoter and cell growth experiments performed to determine if cell growth would be inhibited in samples treated with lysate of the cells containing t Experiments showed a delayed response with differences between those samples treated with lysate from th
    3 KB (395 words) - 19:34, 3 October 2012
  • ...4 ℃ overnight, then we washed the membrane by PBST for three times, each time 10 minutes. In the next step, we applied 2nd antibody for 1 hour at room te <li id='Ref3'>Zhaofeng Li et al, Delayed supplementation of glycine enhances extracellular secretion of the recombin
    10 KB (1,505 words) - 11:35, 5 October 2012
  • ...4 ℃ overnight, then we washed the membrane by PBST for three times, each time 10 minutes. In the next step, we applied 2nd antibody for 1 hour at room te <li id='Ref3'>Zhaofeng Li et al, Delayed supplementation of glycine enhances extracellular secretion of the recombin
    10 KB (1,505 words) - 11:35, 5 October 2012
  • ...4 ℃ overnight, then we washed the membrane by PBST for three times, each time 10 minutes. In the next step, we applied 2nd antibody for 1 hour at room te <li id='Ref3'>Zhaofeng Li et al, Delayed supplementation of glycine enhances extracellular secretion of the recombin
    10 KB (1,505 words) - 11:37, 5 October 2012
  • ...4 ℃ overnight, then we washed the membrane by PBST for three times, each time 10 minutes. In the next step, we applied 2nd antibody for 1 hour at room te <li id='Ref3'>Zhaofeng Li et al, Delayed supplementation of glycine enhances extracellular secretion of the recombin
    10 KB (1,506 words) - 11:40, 5 October 2012
  • ...4 ℃ overnight, then we washed the membrane by PBST for three times, each time 10 minutes. In the next step, we applied 2nd antibody for 1 hour at room te <li id='Ref3'>Zhaofeng Li et al, Delayed supplementation of glycine enhances extracellular secretion of the recombin
    10 KB (1,522 words) - 01:56, 6 October 2012
  • ...4 ℃ overnight, then we washed the membrane by PBST for three times, each time 10 minutes. In the next step, we applied 2nd antibody for 1 hour at room te <li id='Ref3'>Zhaofeng Li et al, Delayed supplementation of glycine enhances extracellular secretion of the recombin
    10 KB (1,522 words) - 01:56, 6 October 2012
  • ...4 ℃ overnight, then we washed the membrane by PBST for three times, each time 10 minutes. In the next step, we applied 2nd antibody for 1 hour at room te <li id='Ref3'>Zhaofeng Li et al, Delayed supplementation of glycine enhances extracellular secretion of the recombin
    10 KB (1,522 words) - 01:57, 6 October 2012
  • <b><h3>Growth Rate Assay</h3></b> ...>Purpose:</u> To assess what effect, if any, our genetic parts have on the growth rate of E.coli.</p>
    5 KB (846 words) - 03:43, 26 November 2014
  • ...he inner membrane of host bacteria at a certain time at an allele-specific time. And then the formation of hole will help Endolysin, a kind of lysozyme, co ...7</sub> is increased relative to S<sub>105</sub>, the 'lysis time' will be delayed<sup>[4]</sup>. The inhibition function of S<sub>107</sub> can be subverted
    7 KB (1,222 words) - 02:51, 18 October 2014
  • ...acially under limited conditions in minimal medium. This could explain the delayed respiration of <a href="http://2014.igem.org/Team:Bielefeld-CeBiTec/Noteboo ...c/Notebook/Media#M9medium">M9 minimal medium</a> with 50 mM glucose. Among growth characteristic we focused on metabolite analysis. Under anaerobic condition
    31 KB (4,871 words) - 19:20, 20 October 2014
  • ...L (1uM) through repeated 1:100 dilutions had no discernable impact on cell growth compared to negative controls (0uM), but had the unexpected effect of minim ...to find a concentration range where cell growth was neither substantially delayed nor was Beta-Galactosidase production only modestly increased. We started
    9 KB (1,421 words) - 03:27, 19 September 2015
  • ...orescence and firstly detecting GFP's fluorescence can represent the delay time which the switch give for responder's expression. ...data and time showed the relationship of the expression of CFP and GFP to time.
    6 KB (933 words) - 12:57, 21 October 2016