Protein domains/Localization

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Certain protein tags can direct a protein to a particular physical location in the cell, such as the nucleus, the membrane, the periplasm, secretion outside of the cell or elsewhere. The localization tags are useful for achieving spatial segregation of proteins.


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-?-NameDescriptionAA sequenceLength
1  BBa_I712009CD4 signal peptide; localizes to plasma membrane 75
1WBBa_I712016myristoilation signal sequence fused to Tobacco Etch Virus (TEV) protease cleavage site 57
1WBBa_I712026myristoilation signal sequence; anchors protein to membrane 21
1  BBa_J32015PelB leader sequence; directs protein to E. coli periplasmic membrane 66
AWBBa_J63007PKI nuclear export sequence; yeast codon optimized 30
1WBBa_J63008SV40 nuclear localization sequence from SV40; yeast codon optimized 21
UWBBa_J64008SicP-SptP chaperone-secretion tag pair from Salmonella type III secretion system 880
1WBBa_K103006OmpA outer membrane protein A fused to linker; displays proteins on cell surface 464
A  BBa_K112230{rbs.pelB} The pelB leader with native rbs; assembly standard 21 85
1  BBa_K125300pilA1 signal sequence from cyanobacterium Synechocystis; secretes protein 62
A  BBa_K125310slr2016 signal sequence from cyanobacterium Synechocystis; secretes protein 53
1  BBa_K128004signal sequence from Lactobacillus bulgaricus; secretes protein 143
A  BBa_K157001EGFR/ErbB1 signal peptide; mediates protein transport to a translocational pore 72
1  BBa_K157010glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac 108
AWBBa_K249004N-terminal Arginine fusion vector 33
AWBBa_K249005C-terminal Arginine Fusion Vector 33
A  BBa_K283036invasin(1-625aa) 1900
A  BBa_K392005yeast SUC2 leader sequence 57
   BBa_K416000Aga2 CDS Responsible for Yeast Surface Display 261
AWBBa_K416003Yeast Secretion Tag 114
A BBa_K416004Aga2:(Gly3Ser)4 Linker Composite Part 314
   BBa_K416008Aga2 fused to eCFP 1835
 WBBa_K678002eGFP-PTS1 for mammalian cells 3398
   BBa_K678025Mitochondrial targeting sequence for Aspergillus nidulans 188
 WBBa_K678033GFP-PTS1 737
 WBBa_K678034mRFP1-NLS, targeted to the nucleus 707
 WBBa_K792002Secretion tag from yeast α-factor mating pheromone (MFα1) 54
   BBa_K801030Polypeptide containing SV40 nuclear localization sequence (SV40 NLS) for nuclear translocation 57
 WBBa_K801039SV40NLS-GAL4AD-linker-PIF3 (part for GAL4/LexA based light-switchable promoter system) 804
 WBBa_K801040SV40NLS-PhyB-linker-Gal4DBD (part for GAL4 based light switchable promoter system) 3294
   BBa_K801041SV40NLS-PhyB-linker-LexA (part for a LexA based light-switchable promoter system) 3459
 WBBa_K801042GAL4 based yeast light-switchable promoter system 6063
   BBa_K801043LexA based yeast light-swithable promoter system 6228
 WBBa_K900002Nucleus Targeting Protein 408
 WBBa_K900003Vacuolar Membrane Targeting Protein 396
 WBBa_K900004Actin Targeting Protein 1779
 WBBa_K900005Plasma Membrane Targeting Sequence 12