1 Registry Star
Plasmid_Backbone
pSB2K3
Part:pSB2K3:Design
Designed by Sri Kosuri Group: Endy Lab, Part Editors, Prather Lab, Registry (2004-05-04)
From partsregistry.org
Inducible copy number BioBrick plasmid
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4404
Illegal BglII site found at 1618
Illegal BglII site found at 2665
Illegal XhoI site found at 3462 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 4404
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4404
Illegal XbaI site found at 4419
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 1061
Illegal NgoMIV site found at 3303
Illegal AgeI site found at 937
Illegal AgeI site found at 2596
Design Notes
- pSCANS via BNL ***
The pSB2 series plasmids are based on the pSCANS-5 plasmid from John Dunn's group at Brookhaven National Laboratory. The pSCANS plasmids are introduced <a href="http://www.genome.bnl.gov/Protocols/pscans.shtml">here</a>.
Source
MIT SBWG modification of pSCANS-5 from BNL
