Part:pSB1AK3

From partsregistry.org

High copy BioBrick assembly plasmid

pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

Annotated APE file for pSB1AK3

Plasmid map for pSB1AK3

Usage and Biology

Kanamycin resistant up to 5000 ug/ml.

Tetracycline sensitive at 1.5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05)

Primers for these Biobrick vectors can be found in Part:BBa_G00100 (aka VF2) and Part:BBa_G00101 (aka. VR). See also Part:BBa_J18907 (aka VR2) for lower temperature sequencing reactions.

This vector is also available in the Fusion (Freiburg) Biobrick format: Part:BBa_J18902

Sequence and Features Content-type: text/html

Software error:

Can't call method "sequence" without a package or object reference at /web-site/partsregistry.org/cgi/lib/AssemblySystem.pm line 202.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

Retrieved from "http://partsregistry.org/Part:pSB1AK3"

[edit]

chassis
copies	100-300
insert	None
mcs	BioBrick
origin	pMB1
resistance	AK