Part:pSB1AK3

From partsregistry.org

High copy BioBrick assembly plasmid

pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

Annotated APE file for pSB1AK3

Plasmid map for pSB1AK3

Usage and Biology

Kanamycin resistant up to 5000 ug/ml.

Tetracycline sensitive at 1.5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05)

Primers for these Biobrick vectors can be found in Part:BBa_G00100 (aka VF2) and Part:BBa_G00101 (aka. VR). See also Part:BBa_J18907 (aka VR2) for lower temperature sequencing reactions.

This vector is also available in the Fusion (Freiburg) Biobrick format: Part:BBa_J18902

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3168
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3174
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3168
Illegal XhoI site found at 1035
Illegal XhoI site found at 2061
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 3168
Illegal suffix found at 2
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3168
Illegal XbaI site found at 3183
Illegal SpeI site found at 2
Illegal PstI site found at 16

chassis
copies	100-300
insert	None
mcs	BioBrick
origin	pMB1
resistance	AK